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Characterization of Stomatin Suppressors ssu-1 AND ssu-2

Carroll, Bryan Thomas
http://rave.ohiolink.edu/etdc/view?acc_num=case1121454937

Abstract Details

2005, Doctor of Philosophy, Case Western Reserve University, Genetics.
Our laboratory is primarily interested in the mechanism of action of volatile anesthetics. We have chosen to use the model system Caenorhabditis elegans to study the genetic determinants of volatile anesthetic sensitivity. Previous work has shown that the function of stomatin-like proteins is one determinant controlling volatile anesthetic response. We isolated two genetic suppressors of the volatile anesthetic-sensitive phenotype resulting from thedeficiency of neuronal stomatin-like protein. This work describes the identification and characterization of these two genetic suppressors, ssu-1 and ssu-2. ssu-1 is the only representation in the C. elegans genome of a type of enzyme involved in sending signals between cells through the circulation. While there are several types of sulfotransferase in C. elegans, ssu-1 is the only cytosolic alcohol sulfotransferase. The cytosolic alcohol sulfotransferase affects the action of signaling molecules by attaching a sulfonate to an alcohol group. Our characterization of this sulfotransferase and its relationship to stomatin-like proteins suggests a common role to the conserved structure of stomatin-like proteins. The second genetic suppressor, ssu-2, is a novel gene that is expressed in seven neurons. The location and neuronal connections of these neurons suggest that they are directly involved in coordinating locomotion, a second phenotype that is altered by stomatin deficiency. Since ssu-2 affects both locomotion and sensitivity to volatile anesthetics, this expression pattern suggests that the same neurons are involved in coordinating locomotion and modifying sensitivity to volatile anesthetics. The functional characterization of ssu-1 and the localization of the expression of ssu-2 offer complementary perspectives into the function of neuronal stomatin-like proteins.
Philip Morgan (Advisor)
131 p.
C. elegans; Caenorhabditis elegans; volatile anesthetic; unc-1; unc-24; ssu-1; ssu-2; stomatin; sulfotransferase; ASJ; RMD; DVA; fls485; sterol

Recommended Citations

Citations

  • Carroll, B. T. (2005). Characterization of Stomatin Suppressors ssu-1 AND ssu-2 [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1121454937

    APA Style (7th edition)

  • Carroll, Bryan. Characterization of Stomatin Suppressors ssu-1 AND ssu-2. 2005. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1121454937.

    MLA Style (8th edition)

  • Carroll, Bryan. "Characterization of Stomatin Suppressors ssu-1 AND ssu-2." Doctoral dissertation, Case Western Reserve University, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=case1121454937

    Chicago Manual of Style (17th edition)

case1121454937
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© 2005, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.