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Bcl-2 Regulates Proapoptotic Calcium Signals by Interacting with the Inositol 1, 4, 5-Trisphosphate Receptor

Rong, Yiping
http://rave.ohiolink.edu/etdc/view?acc_num=case1228322705

Abstract Details

2008, Doctor of Philosophy, Case Western Reserve University, Pharmacology.
Bcl-2 is the founding member of a large family of apoptosis regulating proteins. The antiapoptotic protein Bcl-2 inhibits Ca2+ release from the endoplasmic reticulum (ER). One proposed mechanism involves an interaction of Bcl-2 with the inositol 1, 4, 5-trisphosphate receptor (IP3R) Ca2+ channel localized with Bcl-2 on the ER. Here we document Bcl-2-IP3R interaction within cells by FRET and identify a Bcl-2 interacting region in the regulatory and coupling domain of the IP3R. A peptide (peptide 2) based on this IP3R sequence displaced Bcl-2 from the IP3R and reversed Bcl-2-mediated inhibition of IP3R channel activity in vitro, IP3-induced ER Ca2+ release in permeabilized cells, and cell permeable IP3 ester-induced Ca2+ elevation in intact cells. This peptide also reversed Bcl-2's inhibition of T cell receptor-induced Ca2+ elevation and apoptosis. Furthermore, peptide 2 enhances ABT-737-induced cell death in chronic lymphocytic leukemia cells. The interaction of Bcl-2 with IP3R's contributes to the regulation of proapoptotic Ca2+ signals by Bcl-2. We also investigated the region of Bcl-2 responsible for interaction with the IP3R. Based on results of coimmunoprecipitation and GST pull-down experiments the BH4 domain of Bcl-2 is necessary for interaction with the IP3R. A synthetic peptide corresponding to the BH4 domain of Bcl-2 interacts with the same IP3R domain as full length Bcl-2. TAT-BH4, formed by fusing a peptide corresponding to the BH4 domain of Bcl-2 with the protein transduction domain of HIV TAT, enters cells and functions like full length Bcl-2, to inhibit cytoplasmic Ca2+ elevation and apoptosis induced by T cell receptor (TCR) activation. Two experimental findings establish that these actions of TAT-BH4 are mediated through interaction with the IP3R. First, TAT-BH4 inhibits Ca2+ elevation induced by a cell permeant IP3 ester. Second, peptide 2 that blocks Bcl-2-IP3R interaction reverses the inhibitory effect of TAT-BH4 on both Ca2+ elevation and apoptosis following TCR activation. In summary, these findings indicate that the BH4 domain of Bcl-2 is both necessary and sufficient for interaction with the IP3R. The Bcl-2-IP3R interaction is a potential therapeutic target in diseases associated with Bcl-2's inhibition of cell death.
Clark Distelhorst (Advisor)
Anthony Berdis (Committee Chair)
Shigemi Matsuyama (Committee Member)
William Schilling (Committee Member)
George Dubyak (Committee Member)
229 p.
Biomedical Research; Cellular Biology; Molecular Biology; Oncology; Pharmacology
Bcl-2; Calcium; Ca2+; inositol 1,4,5-trisphosphate receptor; IP3 receptor; IP3R; apoptosis; cell death; T cell receptor activation; BH4; cancer therapy; Bcl-2 inhibitor

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Citations

  • Rong, Y. (2008). Bcl-2 Regulates Proapoptotic Calcium Signals by Interacting with the Inositol 1, 4, 5-Trisphosphate Receptor [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1228322705

    APA Style (7th edition)

  • Rong, Yiping. Bcl-2 Regulates Proapoptotic Calcium Signals by Interacting with the Inositol 1, 4, 5-Trisphosphate Receptor. 2008. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1228322705.

    MLA Style (8th edition)

  • Rong, Yiping. "Bcl-2 Regulates Proapoptotic Calcium Signals by Interacting with the Inositol 1, 4, 5-Trisphosphate Receptor." Doctoral dissertation, Case Western Reserve University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1228322705

    Chicago Manual of Style (17th edition)

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© 2008, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.