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A New Polyacetylenic Alcohol in Fistulina Hepatica: Progress Towards the Identification of Acetylenases in Basidiomycetes

Huffman, Errol A., Sr.
http://rave.ohiolink.edu/etdc/view?acc_num=miami1020271742

Abstract Details

2002, Master of Science, Miami University, Chemistry and Biochemistry.
Cultures of the basidiomycete Fistulina hepatica have been studied to determine the times of appearance of acetylenic natural products. A triacetylenic alcohol has been isolated from the ethereal extract of mycelial cultures of F. hepatica. On the basis of spectroscopic data, its structure was determined to be cis-3-tridec-ene-5,7,9-triyne-1,11-diol. The structure has also been determined for an unsaturated fatty acid, which accumulates when a parsley desaturase is expressed in soybeans. The compound, identified as (14Z)-dehydrocrepenynic acid by NMR and mass spectral data, is believed to be a widely used intermediate in the crepenynate pathway to polyacetylene production in plants and, presumably, fungi. A yeast expression system capable of overexpressing FAD2-type desaturases that may be involved in the biosynthesis of acetylenic natural products was optimized with respect to growth conditions and host cell strain. This system may be suitable for the expression of fungal acetylenases from F. hepatica.
Robert Minto (Advisor)
112 p.
Chemistry, Biochemistry
fatty acid; Crep1; desaturase; YN94-1; HEPATICA

Recommended Citations

Citations

  • Huffman, Sr., E. A. (2002). A New Polyacetylenic Alcohol in Fistulina Hepatica: Progress Towards the Identification of Acetylenases in Basidiomycetes [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1020271742

    APA Style (7th edition)

  • Huffman, Sr., Errol. A New Polyacetylenic Alcohol in Fistulina Hepatica: Progress Towards the Identification of Acetylenases in Basidiomycetes. 2002. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1020271742.

    MLA Style (8th edition)

  • Huffman, Sr., Errol. "A New Polyacetylenic Alcohol in Fistulina Hepatica: Progress Towards the Identification of Acetylenases in Basidiomycetes." Master's thesis, Miami University, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=miami1020271742

    Chicago Manual of Style (17th edition)

miami1020271742
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© 2002, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.