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Glyoxalase 2-2: Over-expression and Characterization of a Metallohydrolase from Arabidopsis thaliana

Wenzel, Nathan F
http://rave.ohiolink.edu/etdc/view?acc_num=miami1069728340

Abstract Details

2003, Master of Science, Miami University, Chemistry and Biochemistry.
In order to investigate the metal binding properties of A. thaliana glyoxalase 2-2, recombinant GLX 2-2 was over-expressed in rich or minimal media containing various amounts of added Zn(II), Fe(II), or Mn(II). The resulting enzymes were purified, characterized, and compared. Kinetic studies and metal analyses of the samples indicated that no significant catalytic differences existed between the enzymes, in spite of the enzymes binding different types and amounts of metal ions. EPR and EXAFS spectroscopies strongly indicated the presence of positive cooperativity of metal binding and of numerous possible dinuclear metal centers, including Fe(III)Zn(II), Fe(III)Fe(II), and Mn(II)Mn(II) centers. The ability to bind various amounts of manganese, iron, and zinc with no apparent change in catalytic activity and the possibility of positive cooperativity in metal binding make A. thaliana glyoxalase 2-2 unique to the family of proteins containing the beta-lactamase fold.
Michael Crowder (Advisor)
96 p.
Chemistry, Biochemistry
glyoxalase; Binuclear metal site; positive cooperativity; Electron paramagnetic resonance; EXAFS

Recommended Citations

Citations

  • Wenzel, N. F. (2003). Glyoxalase 2-2: Over-expression and Characterization of a Metallohydrolase from Arabidopsis thaliana [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1069728340

    APA Style (7th edition)

  • Wenzel, Nathan. Glyoxalase 2-2: Over-expression and Characterization of a Metallohydrolase from Arabidopsis thaliana. 2003. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1069728340.

    MLA Style (8th edition)

  • Wenzel, Nathan. "Glyoxalase 2-2: Over-expression and Characterization of a Metallohydrolase from Arabidopsis thaliana." Master's thesis, Miami University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=miami1069728340

    Chicago Manual of Style (17th edition)

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© 2003, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.