Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


miami1145459719.pdf (1.2 MB)

ETD Abstract Container

Abstract Header

Determining the role of a candidate gene in Drososphila muscle development

Maity, Chaitali
http://rave.ohiolink.edu/etdc/view?acc_num=miami1145459719

Abstract Details

2006, Master of Science, Miami University, Zoology.
In Drosophila, 1424 gene was tagged by the enhancer trapping technique, which involves insertion of a modified transposable element. Reporter expression indicated that the gene is expressed in the CNS and developing flight muscles (IFMs) during pupal stage. This thesis investigates a role for the 1424 gene in the development of flight muscles. Forty- two mutant lines that were generated by genetically excising the P-element were tested. Homozygous mutants were generated as P-element insertion has been reported to be lethal in case of 1424 gene. Upon observation of the lethality during the life –cycle we found the P-element insertion might be semi-lethal as emergence of homozygous 1424 flies was observed. Complementation further support the semi-lethality of the parent strain as nearly all of the mutant lines were able to generate transheterozygotes, but not all of them were able to generate homozygous mutants. Flight test as well as muscle phenotype analysis of the homozygous mutants confirm that this gene represented as 1424 is involved in IFM development.
Joyce Fernandes (Advisor)
70 p.
Enhancer trapping; P-element; Excision lines; IFMs; Flight test

Recommended Citations

Citations

  • Maity, C. (2006). Determining the role of a candidate gene in Drososphila muscle development [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1145459719

    APA Style (7th edition)

  • Maity, Chaitali. Determining the role of a candidate gene in Drososphila muscle development. 2006. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1145459719.

    MLA Style (8th edition)

  • Maity, Chaitali. "Determining the role of a candidate gene in Drososphila muscle development." Master's thesis, Miami University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=miami1145459719

    Chicago Manual of Style (17th edition)

miami1145459719
503
© 2006, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.