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Growth-regulated expression and G0-specific turnover of the mRNA that encodes AH49, a mammalian protein highly related to the mRNA export protein UAP56

Pryor, Anne M
http://rave.ohiolink.edu/etdc/view?acc_num=osu1069259604

Abstract Details

2003, Doctor of Philosophy, Ohio State University, Ohio State Biochemistry Program.
The expression patterns of many genes are regulated at the post-transcriptional level. Therefore, understanding the mechanisms of post-transcriptional processes such as splicing and nuclear export is extremely important. DExD/H box RNA helicases are involved in remodeling RNA-RNA and RNA-protein complexes in all aspects of mRNA metabolism. One DExD/H box protein, UAP56, is a presumed RNA helicase that has been shown to be essential for the nuclear export of all mRNAs in S. cerevisiae and D. melanogaster. I have identified another DExD/H box protein, AH49, which is 89.9% identical to UAP56. Although S. cerevisiae and D. melanogaster have only a single protein corresponding to this helicase (Sub2p and HEL, respectively), I show that mRNAs corresponding to AH49 and UAP56 are both expressed in human and mouse cells. Also, both proteins interact with the export factor Aly in GST pull down assays. UAP56 mRNA was more abundant than AH49 mRNA in many of the human and mouse tissues tested. However, AH49 mRNA was much more abundant than UAP56 mRNA in testes. UAP56 and AH49 mRNAs were present at similar levels in proliferating mouse 3T3 and human WI38 fibroblasts. However, when the cells were induced to enter quiescence, AH49 mRNA levels decreased 3-6-fold while UAP56 mRNA levels remained relatively constant. The amount of AH49 mRNA increased to the level found in proliferating cells within 5 hours after quiescent cells were stimulated to proliferate or following addition of the protein synthesis inhibitor, cycloheximide. AH49 mRNA was relatively unstable (T½ = 4 hr) in quiescent cells but was stabilized immediately following growth stimulation or addition of cycloheximide. In contrast, there was little change in the content or stability of UAP56 mRNA following growth stimulation. These observations show that two related RNA helicases, UAP56 and AH49, have differential gene regulation. The reason for this differential regulation may be because the two proteins have unique functions and remains to be determined.
Lee Johnson (Advisor)
187 p.
Chemistry, Biochemistry
UAP56; AH49; mRNA stability; DExD/H box RNA helicase; mRNA splicing and export

Recommended Citations

Citations

  • Pryor, A. M. (2003). Growth-regulated expression and G0-specific turnover of the mRNA that encodes AH49, a mammalian protein highly related to the mRNA export protein UAP56 [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1069259604

    APA Style (7th edition)

  • Pryor, Anne. Growth-regulated expression and G0-specific turnover of the mRNA that encodes AH49, a mammalian protein highly related to the mRNA export protein UAP56. 2003. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1069259604.

    MLA Style (8th edition)

  • Pryor, Anne. "Growth-regulated expression and G0-specific turnover of the mRNA that encodes AH49, a mammalian protein highly related to the mRNA export protein UAP56." Doctoral dissertation, Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=osu1069259604

    Chicago Manual of Style (17th edition)

osu1069259604
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© 2003, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.