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Detection and molecular characterization of porcine noroviruses and sapoviruses

Wang, Qiuhong
http://rave.ohiolink.edu/etdc/view?acc_num=osu1117555338

Abstract Details

2005, Doctor of Philosophy, Ohio State University, Veterinary Preventive Medicine.
Noroviruses (NoV) and sapoviruses (SaV) are emerging pathogens that cause gastroenteritis in humans and animals. Whether NoVs and SaVs are zoonotic pathogens is unclear due to limited studies of animal NoVs and SaVs. We screened for porcine NoVs by reverse transcription-PCR (RT-PCR) on fecal samples from US pigs. Based on sequence analysis of the 3 kb of the genomes of 5 porcine NoVs, 3 genotypes within genogroup II (GII) and two potential recombinant strains were identified. One genotype of porcine NoVs was genetically and antigenically related to human NoVs and replicated in gnotobiotic pigs. By similar approaches, porcine SaVs were identified as genetically diverse viruses comprising at least 2 genogroups. Two porcine SaVs were potential recombinants. One porcine SaV strain (Po/SaV/MI-QW19/2002/US) was genetically most closely related to human SaVs based on the partial RdRp sequence (286 nt). Furthermore, several primer pairs were designed and evaluated for the detection of porcine NoVs and SaVs, respectively. An internal control RNA was developed and used in RT-PCR to monitor for RT-PCR inhibition. Microwell hybridization assays were developed to confirm the porcine NoV- and SaV-specific amplicons. Finally, a prevalence study of porcine NoVs and SaVs in US swine was performed by the newly developed RT-PCR-hybridization assays. The porcine NoVs were detected exclusively from finisher pigs in 4 of 7 farms and 1 slaughterhouse with an overall prevalence of 20% in finisher pigs. Porcine SaVs were detected from all ages of pigs. The prevalence of porcine SaVs was 62% overall, lowest in nursing pigs (21%) and highest in post-weaning pigs (83%). Mixed infections of NoVs and SaVs were common in finisher pigs with an overall prevalence of 27% among NoV- or SaV-positive pigs. These findings have improved our understanding of the genetic diversity of porcine NoVs and SaVs and their relationships to human strains. Certain porcine NoVs and SaVs are genetically or antigenically related to human strains. This finding and the high prevalence of NoVs and SaVs in subclinically infected swine including their detection from slaughterhouse pigs increase the risks that pigs may be reservoirs for human NoV and SaV strains.
Linda Saif (Advisor)
251 p.
norovirus; sapovirus; porcine; genetic classification; recombinant; prevalence; internal control RNA; microwell hybridization

Recommended Citations

Citations

  • Wang, Q. (2005). Detection and molecular characterization of porcine noroviruses and sapoviruses [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1117555338

    APA Style (7th edition)

  • Wang, Qiuhong. Detection and molecular characterization of porcine noroviruses and sapoviruses. 2005. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1117555338.

    MLA Style (8th edition)

  • Wang, Qiuhong. "Detection and molecular characterization of porcine noroviruses and sapoviruses." Doctoral dissertation, Ohio State University, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=osu1117555338

    Chicago Manual of Style (17th edition)

osu1117555338
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© 2005, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.