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PROTEOMIC ANALYSIS OF THE EFFECTS OF DIET IN ZEBRAFISH LIVER

Jury, David R.
http://rave.ohiolink.edu/etdc/view?acc_num=akron1129389691

Abstract Details

2005, Master of Science, University of Akron, Biology.
Characterizing molecular signaling in lipid metabolism traditionally has not been investigated with an integrated global mechanism approach. The peroxisome proliferated-activated receptors (PPARs) are a family of transcription factors that in response to lipids orchestrate metabolism. It was hypothesized that a high-calorie diet in zebrafish would up-regulate activity of PPARs. Proteomics identified discrete focused bands with greater than a 200% difference between the high-calorie and low-calorie diet treatment groups. Protein bands were subsequently submitted for identification with mass spectrometry. A lower total number of discrete proteins were expressed in the high-calorie group, which can be interpreted as part of a general stress response. Specific proteins up-regulated indicative of stress were prohibitin, glutamate oxaloacetate transaminase, and glyoxylase II. Down-regulated proteins indicative of stress induced retarded growth included cytokeratin, brain type fatty acid binding protein, profilin 2, and histone. Other proteins exhibited expression patterns that did not fit into a predictable stress response.
Richard Londraville (Advisor)
54 p.
Proteomics; Diet; Zebrafish; Liver; Calorie availability; Stress response

Recommended Citations

Citations

  • Jury, D. R. (2005). PROTEOMIC ANALYSIS OF THE EFFECTS OF DIET IN ZEBRAFISH LIVER [Master's thesis, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1129389691

    APA Style (7th edition)

  • Jury, David. PROTEOMIC ANALYSIS OF THE EFFECTS OF DIET IN ZEBRAFISH LIVER. 2005. University of Akron, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1129389691.

    MLA Style (8th edition)

  • Jury, David. "PROTEOMIC ANALYSIS OF THE EFFECTS OF DIET IN ZEBRAFISH LIVER." Master's thesis, University of Akron, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=akron1129389691

    Chicago Manual of Style (17th edition)

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© 2005, all rights reserved.
This open access ETD is published by University of Akron and OhioLINK.