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Sry1 decreases urinary sodium excretion in the kidney of male wistar kyoto rats

Hart, Michael
http://rave.ohiolink.edu/etdc/view?acc_num=akron1195232446

Abstract Details

2007, Master of Science, University of Akron, Biology.
Our lab has identified a locus on the Y chromosome of the spontaneously hypertensive rat that is associated with a 20-25 mmHg increase in blood pressure (BP). A candidate gene for this locus is Sry1, also known as the testis determining factor. We have shown that Sry1 inserted into a mammalian expression vector and electroporated into the kidney of the normotensive Wistar-Kyoto rat (WKY), produced a 50% increase in plasma norepinephrine content after two weeks compared to empty vector controls. The aim of the current research was to investigate if exogenous Sry1 would decrease glomerular filtration rate and urinary Na+ excretion in the WKY strain and secondly if those effects were dependent upon the androgen receptor. Age-matched WKY adult male rats were electroporated with either the Sry1 expression construct or an empty vector and kidney function evaluated by 24 hr creatinine clearance, urinary electrolytes and albuminuria. BP and plasma catecholamines were measured to assess sympathetic nervous activity. In addition, flutamide was administered to a sub-group of rats that received Sry1 to determine the potential involvement of testosterone and/or its receptor. At 21 days post-electroporation the Sry1 group had an average increase in BP of 14 mmHg (p<0.001), a 65% increase in plasma norepinephrine (p=0.001), a 58% decrease in albuminuria (p<0.05), a decrease in urinary Na+ excretion of 43% (p=0.01) and no difference in 24 hr creatine clearance compared to empty vector controls. In addition, the administration of flutamide ameliorated the decrease in urinary Na+ found in the Sry1 group. Despite an increase in BP, which increases pressure natriuresis, the Sry1 group retained more Na+ than empty vector controls. Our results are consistent with the idea that one function of Sry1 may be the renal regulation of Na+ with a potential dependence on testosterone and/or its receptor for full effect.
Daniel Ely (Advisor)
51 p.
Biology, Animal Physiology
Sry; catecholamine; norepinephrine; albuminuria; electroporation; testosterone; androgen receptor.

Recommended Citations

Citations

  • Hart, M. (2007). Sry1 decreases urinary sodium excretion in the kidney of male wistar kyoto rats [Master's thesis, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1195232446

    APA Style (7th edition)

  • Hart, Michael. Sry1 decreases urinary sodium excretion in the kidney of male wistar kyoto rats. 2007. University of Akron, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1195232446.

    MLA Style (8th edition)

  • Hart, Michael. "Sry1 decreases urinary sodium excretion in the kidney of male wistar kyoto rats." Master's thesis, University of Akron, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=akron1195232446

    Chicago Manual of Style (17th edition)

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© 2007, all rights reserved.
This open access ETD is published by University of Akron and OhioLINK.