Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


akron1207860116.pdf (746.06 KB)

ETD Abstract Container

Abstract Header

Organic Phase Entrapment of Glucose Oxidase In Polymeric Nanoparticles

Hancock, James
http://rave.ohiolink.edu/etdc/view?acc_num=akron1207860116

Abstract Details

2008, Master of Science, University of Akron, Chemical Engineering.
Immobilizing enzymes using polymeric particles is an excellent means to increase the reusability of a biocatalyst. Recovering active enzyme free in solution is difficult. However, the immobilized enzyme-particle system can be easily removed using methods such as filtration or centrifugation. One interesting immobilization technique is to entrap enzymes within the polymer matrix. To achieve this, polystyrene particles of varied size (100 to 750 nm) and degree of cross-linking (monomer: cross-linker molar ratios ranging from 1: 1 to systems with no cross-linker) were treated with glucose oxidase in toluene solution. In toluene, the polymer expands, allowing enzyme to diffuse into the matrix. Then, the particles are treated with hexane, a worse solvent, which forces the polymer to shrink, entrapping the enzyme. The enzyme-polymer particle complexes were tested using a standard enzyme activity assay to quantify the various systems of varied size and cross-linking. The effect of cross-linking appears to be that there is a minimum level at about 0.1 moles/mole required to support reaction, which had an observed reaction rate of about 65 micro moles per min per mg solid. Kinetic studies at varied polymer particle size indicate that smaller particles result in higher reaction rates. Kinetic studies at varied polymer particle size indicate that smaller particles result in higher reaction rates; for the case of 250 nm particles, the observed rate was close to 30 micro moles per min per mg solid.
Ping Wang, PhD (Advisor)
Lu-Kwang Ju, PhD (Committee Member)
Edward Evans, PhD (Committee Member)
75 p.
Biochemistry; Chemical Engineering; Engineering; Particle Physics; Polymers
nanoparticle; nanotechnology; enzyme immobilization; enzyme entrapment; enzyme kinetics; polymer swelling

Recommended Citations

Citations

  • Hancock, J. (2008). Organic Phase Entrapment of Glucose Oxidase In Polymeric Nanoparticles [Master's thesis, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1207860116

    APA Style (7th edition)

  • Hancock, James. Organic Phase Entrapment of Glucose Oxidase In Polymeric Nanoparticles. 2008. University of Akron, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1207860116.

    MLA Style (8th edition)

  • Hancock, James. "Organic Phase Entrapment of Glucose Oxidase In Polymeric Nanoparticles." Master's thesis, University of Akron, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=akron1207860116

    Chicago Manual of Style (17th edition)

akron1207860116
763
© 2008, all rights reserved.
This open access ETD is published by University of Akron and OhioLINK.