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Protocadherin-17 Function in Zebrafish Retina Development

Chen, Yun
http://rave.ohiolink.edu/etdc/view?acc_num=akron1352293381

Abstract Details

2012, Doctor of Philosophy, University of Akron, Integrated Bioscience.
Expression and function of protocadherin-17 (pcdh17) in zebrafish retinal development were analyzed in this study. Pcdh17 mRNA (pcdh17) expression pattern was characterized using whole mount in situ hybridization method. Antisense morphlino oligonucleotides (MOs) technique was used to determine pcdh17 function. Moreover, molecular mechanisms underlying pcdh17 expression and function were studied using proteomics. pcdh17 was expressed in developing zebrafish retina during critical stages of its development. Abnormal eye and retinal development was observed in developing zebrafish (49-72 hours post fertilization, hpf) injected with zebrafish pcdh17 specific MOs (pcdh17 morphants). The morphants had significantly smaller eyes and disrupted differentiated retinal cells (e.g. retinal ganglion cells and photoreceptors), due mainly to decreased cell proliferation as well as defects of nonneuronal cell differentiation. Proteomic analysis revealed that several hundred proteins were differentially expressed between wildtype embryos and pcdh17 morphants. A subset of spots showing the biggest differences were identified using Mass Spectrometry. These proteins included phosphoglycerate kinase, beta-actin-like protein, and glial fibrillary acidic protein (GFAP). Most of the identified proteins are involved in basic cellular metabolism and cellular structure, whereas GFAP is a molecule involved in Notch signaling pathway known to play a critical role in vertebrate retinal development. In addition, pcdh17 functions in retina development via regulating certain transcription factors, classical cadherins, homophilic reacting molecules and integrin-dependent adhesion. My research suggests that pcdh17 plays an important role in zebrafish retinal development, likely through involving multiple pathways including Notch-Delta, Wnt pathways.
Qin Liu, Dr. (Advisor)
Richard Londraville, Dr. (Committee Member)
Brian Bagatto, Dr. (Committee Member)
Bruce Cushing, Dr. (Committee Member)
Zhonghui Duan, Dr. (Committee Member)
87 p.
Biology
Protocadherin-17; Zebrafish; Retina development; Molecular mechanisms

Recommended Citations

Citations

  • Chen, Y. (2012). Protocadherin-17 Function in Zebrafish Retina Development [Doctoral dissertation, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1352293381

    APA Style (7th edition)

  • Chen, Yun. Protocadherin-17 Function in Zebrafish Retina Development. 2012. University of Akron, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1352293381.

    MLA Style (8th edition)

  • Chen, Yun. "Protocadherin-17 Function in Zebrafish Retina Development." Doctoral dissertation, University of Akron, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=akron1352293381

    Chicago Manual of Style (17th edition)

akron1352293381
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© 2012, some rights reserved.Creative Commons License Protocadherin-17 Function in Zebrafish Retina Development by Yun Chen is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by University of Akron and OhioLINK.