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A Spin-Coated Thermoresponsive Substrate for Rapid Cell Sheet Detachment and Its Applications in Cardiac Tissue Engineering

Patel, Nikul Girishkumar
http://rave.ohiolink.edu/etdc/view?acc_num=akron1386671454

Abstract Details

2014, Doctor of Philosophy, University of Akron, Biomedical Engineering.
A modular approach to create organized tissue that has gained much attention since the introduction of thermoresponsive surfaces is the assembly of cell sheets. With a change in temperature of grafted poly-N-isopropylacrylamide (pNIPAAm), a thermoresponsive polymer, cell sheets can be harvested with their deposited extracellular matrix (ECM) intact. PNIPAAm has been covalently grafted to cell culture substrates by two primary methods: electron beam irradiation and plasma polymerization. Most tissue engineering laboratories have difficulties using these approaches to custom-make their thermoresponsive surface for specific applications due to the complexities of associated procedures and limited access to required equipment (e.g. e-beam). The goal of this study was to develop a simple, cost-effective approach for the creation of thermoresponsive surfaces using commercially available pNIPAAm for cell sheet harvest. Methods to effectively manipulate viable cell sheets (e.g. transfer and stack) were developed. In addition, a study of cell sheet interaction on fibrin gels is presented and analyzed for future cardiac tissue engineering applications.
Ge Zhang, Dr. (Advisor)
Bi-min Zhang Newby, Dr. (Advisor)
Rebecca Willits, Dr. (Committee Member)
Bing Yu, Dr. (Committee Member)
Richard Londraville, Dr. (Committee Member)
127 p.
Biomedical Engineering; Biomedical Research
rapid cell sheet detachment; thermally responsive material; Poly N-isopropylacrylamide; pNIPAAm; aminopropyltriethoxysilane; APTES; human mesenchcymal stem cells; MSC; matrix metalloproteinase; MMP; fibrin; infarct

Recommended Citations

Citations

  • Patel, N. G. (2014). A Spin-Coated Thermoresponsive Substrate for Rapid Cell Sheet Detachment and Its Applications in Cardiac Tissue Engineering [Doctoral dissertation, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1386671454

    APA Style (7th edition)

  • Patel, Nikul. A Spin-Coated Thermoresponsive Substrate for Rapid Cell Sheet Detachment and Its Applications in Cardiac Tissue Engineering. 2014. University of Akron, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1386671454.

    MLA Style (8th edition)

  • Patel, Nikul. "A Spin-Coated Thermoresponsive Substrate for Rapid Cell Sheet Detachment and Its Applications in Cardiac Tissue Engineering." Doctoral dissertation, University of Akron, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=akron1386671454

    Chicago Manual of Style (17th edition)

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This open access ETD is published by University of Akron and OhioLINK.