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Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers

Chen, Xi
http://orcid.org/0000-0002-1070-8024
http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122

Abstract Details

2015, Master of Science, University of Akron, Polymer Science.
Biomimetic catalysis has great impact on the development of the research of organic catalysis1. Immobilized enzyme used as catalysts has attracted a lot of attention because of its high stability and the excellent ability of recovering from the reaction environment. Furthermore, the activity of immobilized enzyme can be retained after long-term storage or exposure to high temperatures2,3. In this study, Immobilization of GOx was achieved by adsorption and encapsulation of GOx onto porous polymer support, and catalytic activity of GOx immobilized porous support was evaluated by testing the formation of H2O2. On the other hand, biological sequestration of carbon dioxide (CO2) is one of the proposed approaches for CO2 unitization. Carbonic anhydrase was chose as catalyst to study the catalytic reaction of CO2. The reaction was in situ monitored by Attenuated total reflectance (ATR), the preliminary results indicated the formation of bicarbonate (HCO3-), which was evidenced by the IR characteristic band at 1400 cm-1 4.
Steven S.C. Chuang, Dr. (Advisor)
Toshikazu Miyoshi, Dr. (Committee Member)
105 p.
Polymers
Enzyme, immobilization, UV-vis, infrared

Recommended Citations

Citations

  • Chen, X. (2015). Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers [Master's thesis, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122

    APA Style (7th edition)

  • Chen, Xi. Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers. 2015. University of Akron, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122.

    MLA Style (8th edition)

  • Chen, Xi. "Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers." Master's thesis, University of Akron, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122

    Chicago Manual of Style (17th edition)

akron1428327122
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This open access ETD is published by University of Akron and OhioLINK.