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Effect of Surface Functional Groups on Chondrocyte Behavior Using Molecular Gradients

Li, Qiyao
http://rave.ohiolink.edu/etdc/view?acc_num=akron1428399167

Abstract Details

2015, Master of Science, University of Akron, Polymer Science.
Recently, efforts addressing osteoarthritis (OA) have focused on preserving the host joint by addressing the underlying mechanical changes in cartilage during OA progression. There are the limitations to using OA chondrocytes including low numbers and the metabolic imbalance between expressions of catabolic matrix cytokines. For progression to be successful, it is necessary for implants to develop optimal surface conditions to facilitate expansion of cell number and bioactivity while preserving the narrow cellular phenotype. Initial studies have shown that competitive surface-engineering approaches such as soluble protein signaling factors and surface chemistry system have a profound effect on lineage commitment and cellular function. Collectively, functionalized amine and hydroxyl surfaces have been studied and found to promote stem cell viability, cell adhesion and migration. Unfortunately, chemical concentrations have not been widely examined even though the cell behavior has been shown strongly influenced by the concentration of chemical functionality. Herein we examined the effects of surface functional group concentrations, examing chondrocyte proliferation and phenotype maintenance within continuously variable one-dimensional methyl/amine/hydroxyl concentration gradients. The gradients were fabricated using the “vacuum away” confined channel vapor deposition method which is highly tunable and versatile. Cell number and phenotype maintenance were found to vary along the chemical gradients. Thus spatial presentation of surface chemistry shows a significant impact in modulating human osteoarthritic chondrocyte behavior.
Matthew Becker, Dr. (Advisor)
Abraham Joy, Dr. (Committee Member)
90 p.
Materials Science; Polymer Chemistry

Recommended Citations

Citations

  • Li, Q. (2015). Effect of Surface Functional Groups on Chondrocyte Behavior Using Molecular Gradients [Master's thesis, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1428399167

    APA Style (7th edition)

  • Li, Qiyao. Effect of Surface Functional Groups on Chondrocyte Behavior Using Molecular Gradients. 2015. University of Akron, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1428399167.

    MLA Style (8th edition)

  • Li, Qiyao. "Effect of Surface Functional Groups on Chondrocyte Behavior Using Molecular Gradients." Master's thesis, University of Akron, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=akron1428399167

    Chicago Manual of Style (17th edition)

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© 2015, all rights reserved.
This open access ETD is published by University of Akron and OhioLINK.