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AlalwiatAhlamfinal..pdf (8.69 MB)
ETD Abstract Container
Abstract Header
Mass Spectrometry Methods for the Analysis of Biodegradable Hybrid Materials
Author Info
Alalwiat, Ahlam Adnan
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=akron1434894354
Abstract Details
Year and Degree
2015, Doctor of Philosophy, University of Akron, Chemistry.
Abstract
This dissertation focuses on the characterization of hybrid materials and surfactant blends by using mass spectrometry (MS), tandem mass spectrometry (MS/MS), liquid chromatography (LC), and ion mobility (IM) spectrometry combined with measurement and simulation of molecular collision cross sections. Chapter II describes the principles and the history of mass spectrometry (MS) and liquid chromatography (LC). Chapter III introduces the materials and instrumentation used to complete this dissertation. In chapter IV, two hybrid materials containing poly(t-butyl acrylate) (PtBA) or poly(acrylic acid) (PAA) blocks attached to a hydrophobic peptide rich in valine and glycine (VG2), as well as the poly(acrylic acid) (PAA) and VG2 peptide precursor materials, are characterized by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), electrospray ionization mass spectrometry (ESI-MS) and ion mobility mass spectrometry (IM-MS). Collision cross-sections and molecular modeling have been used to determine the final architecture of both hybrid materials. Chapter V investigates a different hybrid material, [BMP-2(HA)2], comprised of a dendron with two polyethylene glycol (PEG) branches terminated by a hydroxyapatite binding peptide (HA), and a focal point substituted with a bone morphogenic protein mimicking peptide (BMP-2). MALDI-MS, ESI-MS and IM-MS have been used to characterize the HA and BMP-2 peptides. Collisionally activated dissociation (CAD) and electron transfer dissociation (ETD) have been employed in double stage (i.e. tandem) mass spectrometry (MS/MS) experiments to confirm the sequences of the two peptides HA and BMP-2. The MALDI-MS, ESI-MS and IM-MS methods were also applied to characterize the [BMP-2(HA)2] hybrid material. Collision cross-section measurements and molecular modeling indicated that [BMP-2(HA)2] can attain folded or extended conformation, depending on its degree of protonation (charge state). Chapter VI focuses on the analysis of alkyl polyglycoside (APG) surfactants by MALDI-MS and ESI-MS, MS/MS, and by combining MS and with ion mobility (IM) and/or ultra-performance liquid chromatography (UPLC) separation in LC-IM and LC-IM-MS experiments. Chapter VII summaries this dissertation’s findings.
Committee
Chrys Wesdemiotis, Dr. (Advisor)
Leah Shriver, Dr. (Committee Member)
Adam Smith, Dr. (Committee Member)
Sailaja Paruchuri, Dr. (Committee Member)
Matthew Becker, Dr. (Committee Member)
Pages
324 p.
Subject Headings
Analytical Chemistry
;
Chemistry
Keywords
Mass Spectrometry
;
the Analysis of Biodegradable Hybrid Materials
;
Hybrid Materials
Recommended Citations
Refworks
EndNote
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Citations
Alalwiat, A. A. (2015).
Mass Spectrometry Methods for the Analysis of Biodegradable Hybrid Materials
[Doctoral dissertation, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1434894354
APA Style (7th edition)
Alalwiat, Ahlam.
Mass Spectrometry Methods for the Analysis of Biodegradable Hybrid Materials.
2015. University of Akron, Doctoral dissertation.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=akron1434894354.
MLA Style (8th edition)
Alalwiat, Ahlam. "Mass Spectrometry Methods for the Analysis of Biodegradable Hybrid Materials." Doctoral dissertation, University of Akron, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=akron1434894354
Chicago Manual of Style (17th edition)
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Document number:
akron1434894354
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Copyright Info
© 2015, all rights reserved.
This open access ETD is published by University of Akron and OhioLINK.