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Vesicle-Protein Diffusion and Interaction Study Using Time Resolved Fluorescence Correlation Spectroscopy

Rouhvand, Bahar
http://rave.ohiolink.edu/etdc/view?acc_num=akron1503261462042903

Abstract Details

2017, Master of Science, University of Akron, Physics.
Fluorescence correlation spectroscopy (FCS) is a useful experimental technique, that uses statistical analysis of fluorescence intensity fluctuations and a correlation function. The method permits studying the kinetics and interactions of particles not only on the surface of the solution, but also in the bulk. Another benefit of FCS is that it does not require the selection of specific molecules or time intervals for its measurements. By analyzing biological dynamics, we can learn about concentration, viscosity, individual movement and diffusion of particles as well as interactions and the possibility of binding between them. In this work, we present a brief explanation on the analytic formalism of the governing concepts of FCS, as well as detail the experimental setups. We also discuss protein-vesicle interactions and the kinematics of double-stranded DNA and labeled nucleotides. The brightness and size of molecules, before and after adding vesicles to the protein solution, are calculated. Finally the possibility of vesicle-protein binding is validated.
Adam Smith, Dr. (Advisor)
Ben Hu, Dr. (Committee Chair)
Jutta Luettmer-Strathmann, Dr. (Committee Member)
57 p.
Biochemistry; Biophysics; Molecular Biology; Molecular Chemistry; Physical Chemistry; Scientific Imaging
Fluorescence spectroscopy; DLS; Vesicle; FITC; Fluorescence; Correlation function; Cross correlation; Auto correlation; Lipid; bilayer; Binding; Double-stranded DNA; Lipid-polymer interactions; Brownian diffusion; Lipid mobility;

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Citations

  • Rouhvand, B. (2017). Vesicle-Protein Diffusion and Interaction Study Using Time Resolved Fluorescence Correlation Spectroscopy [Master's thesis, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1503261462042903

    APA Style (7th edition)

  • Rouhvand, Bahar. Vesicle-Protein Diffusion and Interaction Study Using Time Resolved Fluorescence Correlation Spectroscopy. 2017. University of Akron, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1503261462042903.

    MLA Style (8th edition)

  • Rouhvand, Bahar. "Vesicle-Protein Diffusion and Interaction Study Using Time Resolved Fluorescence Correlation Spectroscopy." Master's thesis, University of Akron, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=akron1503261462042903

    Chicago Manual of Style (17th edition)

akron1503261462042903
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© 2017, all rights reserved.
This open access ETD is published by University of Akron and OhioLINK.