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NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY IN THE STUDY OF PROTEIN-LIGAND INTERACTIONS

Morris, Daniel L
http://orcid.org/0000-0001-5274-0172
http://rave.ohiolink.edu/etdc/view?acc_num=akron1524681449524557

Abstract Details

2018, Doctor of Philosophy, University of Akron, Chemistry.
Numerous nuclear magnetic resonance (NMR) experiments for observing protein-ligand interactions have been proposed over the years since NMR was first used to study biomolecules. In the work that follows, a survey of the most widely used methods is presented with applications to three protein systems. First, orthologous glutaredoxins from homo sapiens and some bacterial species were compared structurally to assay their binding interactions in a fragment-based drug discovery project. This analysis yielded ortholog specific binders to be considered as antibiotic drug leads against the glutaredoxins from Brucella melitensis and Pseudomonas aeruginosa. Next, a pedagogical research initiative was conducted along with an undergraduate biochemistry laboratory course to describe the folding and iron-sulfur cluster binding properties of the mitochondrial membrane protein mitoNEET. The students created several mitoNEET mutants and used 1D and 2D NMR experiments to characterize their effect on mitoNEET’s structure and ligand binding capabilities. Finally, the lysozyme isolated from hen egg whites was observed catalyzing the polymerization of a polyacetylene. This marked the first time a hydrolase was observed catalyzing a synthetic polymer. NMR, along with X-ray crystallography, was used to describe the reaction mechanism, kinetics, and properties of the polymer product.
Christopher Ziegler, Dr. (Advisor)
Leah Shriver, Dr. (Committee Member)
Sailaja Paruchuri, Dr. (Committee Member)
Aliaksei Boika, Dr. (Committee Member)
Abraham Joy, Dr. (Committee Member)
272 p.
Biochemistry; Bioinformatics; Biology; Biophysics; Cellular Biology; Chemistry; Medicine; Microbiology; Molecular Biology; Molecular Chemistry; Molecules; Organic Chemistry; Pedagogy; Pharmaceuticals
NMR; X-ray crystallography; protein; FBDD; glutaredoxin, mitoNEET; lysozyme

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Citations

  • Morris, D. L. (2018). NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY IN THE STUDY OF PROTEIN-LIGAND INTERACTIONS [Doctoral dissertation, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1524681449524557

    APA Style (7th edition)

  • Morris, Daniel. NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY IN THE STUDY OF PROTEIN-LIGAND INTERACTIONS. 2018. University of Akron, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1524681449524557.

    MLA Style (8th edition)

  • Morris, Daniel. "NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY IN THE STUDY OF PROTEIN-LIGAND INTERACTIONS." Doctoral dissertation, University of Akron, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=akron1524681449524557

    Chicago Manual of Style (17th edition)

akron1524681449524557
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© 2018, all rights reserved.
This open access ETD is published by University of Akron and OhioLINK.