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Searching for the Rosetta Stones in the Multifunctional Proteins of the Phytophthora Sojae Genome

Wittenschlaeger, Thomas M, II
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1181012189

Abstract Details

2007, Master of Science (MS), Bowling Green State University, Biological Sciences.
Defining protein-proteins interactions is a major challenge in newly sequenced genomes. Novel multifunctional proteins in oomycete genomes may be useful in assembling both metabolic and regulatory pathways. A global survey of the oomycete Phytophthora sojae genome, identified 274 novel multifunctional proteins using strict criteria that excluded multiexonic gene models without EST support. These P. sojae proteins have significant BLAST hits to two or more different proteins. Such proteins have been posited as Rosetta stones, since their association in one genome has been used to infer the association of orthologous proteins in other genomes. In our analysis, we adopted the reciprocal smallest distance algorithm (Wall et al 2003) to identify potential orthologs in 34 sequenced genomes. Surprisingly, this approach identified only ten potential Rosetta stones, where each domain of a multifunctional protein had an ortholog in the same organism. The evolutionary history of oomycetes involved the endosymbiotic acquisition of a red algae, and subsequent transfer of nuclear and plastid genes to the host nucleus. We postulate that this endosymbiotic event (genome acquisition and recombination) has enabled the ancestral genome to develop metabolic and regulatory pathways that are distinct from those of the animal, fungal, and plant genomes. A separate phylogenetic analysis of four metabolic multifunctional proteins suggested in each case that the domains within these multifunctional proteins were more closely related to proteins from different phylogenetic groups. This suggests that such proteins arose from fusion events of proteins acquired by horizontal transfer from the algal endosymbiont or bacterial genomes. Our observations suggest that this evolutionary strategy of genome acquisition and recombination should also be assessed in other members of the Chromalveolates.
Paul Morris (Advisor)
31 p.
PROTEINS; Zn-finger; FYVE type; Calcium-binding EF-hand

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Citations

  • Wittenschlaeger, II, T. M. (2007). Searching for the Rosetta Stones in the Multifunctional Proteins of the Phytophthora Sojae Genome [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1181012189

    APA Style (7th edition)

  • Wittenschlaeger, II, Thomas. Searching for the Rosetta Stones in the Multifunctional Proteins of the Phytophthora Sojae Genome. 2007. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1181012189.

    MLA Style (8th edition)

  • Wittenschlaeger, II, Thomas. "Searching for the Rosetta Stones in the Multifunctional Proteins of the Phytophthora Sojae Genome." Master's thesis, Bowling Green State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1181012189

    Chicago Manual of Style (17th edition)

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© 2007, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.