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bgsu1182462962.pdf (1.63 MB)
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P1 Bacteriophage and Tol System Mutants
Author Info
Smerk, Cari L
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1182462962
Abstract Details
Year and Degree
2007, Master of Science (MS), Bowling Green State University, Biological Sciences.
Abstract
Mutations affecting the Tol system of Escherichia coli render the cells resistant to bacteriophage P1 by blocking phage maturation. Mutations affecting the energy harvesting complex component, TolQ, were resistant to lysis by bacteriophage P1. Characterization of specific Tol system mutants suggested that phage maturation was not coupled to energy transduction and is unique to TolQ. This study aims to identify the role that the TolQ protein plays in the phage maturation process. Strains of cells were inoculated with P1 and phage production determined using one step growth curves. Strains lacking the TolQ protein rendered P1 unable to produce the burst of progeny phage after a single generation. A strain containing an energetically inactive TolQ protein are able to produce enough viable progeny in one generation of phage to constitute a burst. This data suggests that there is some unique function of TolQ is parasitized by P1 to mature and produce viable progeny.
Committee
Ray Larsen (Advisor)
Pages
67 p.
Keywords
phage
;
TolQ
;
P1
;
BACTERIOPHAGE
;
TOL SYSTEM
;
TOL
;
PFU
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Citations
Smerk, C. L. (2007).
P1 Bacteriophage and Tol System Mutants
[Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1182462962
APA Style (7th edition)
Smerk, Cari.
P1 Bacteriophage and Tol System Mutants.
2007. Bowling Green State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1182462962.
MLA Style (8th edition)
Smerk, Cari. "P1 Bacteriophage and Tol System Mutants." Master's thesis, Bowling Green State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1182462962
Chicago Manual of Style (17th edition)
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Document number:
bgsu1182462962
Download Count:
1,910
Copyright Info
© 2007, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.