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Design and Development of Metal-Peptide Nanoscaled Materials

Tsurkan, Mikhail V
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1182799840

Abstract Details

2007, Doctor of Philosophy (Ph.D.), Bowling Green State University, Photochemical Sciences.
The work presented in this dissertation demonstrates how de-novo designed peptides having the double stranded á-helical coiled-coil conformation can be organized into molecular assemblies by metal coordination. The design of these peptides explores the positioning of a strong metal binding site, 4-pyridylalanine, within the hydrophilic portion of the peptides in order to coordinate the metal complexes into well-defined geometries. The effect of the peptide’s sequence on the stability of the formed metal-peptide complex has been shown through the reaction of Pal14 and Pal14n with ethylenediamine platinum (II). In particular, it was found that peptides based on an IEALEGK repeat do not form stable metals complexes. In contrast, peptides based on an IAALEQK repeat do form stable platinum complexes which in water assemble into globular structures that are 40 nm in diameter. Incorporation of a covalent crosslink into the Pal14n coiled-coil structure (Pal14C19nox) forces this peptide to form linear assemblies after the reaction with the platinum complex. The last chapter of this dissertation reveals that multiple metal binding sites on the hydrophilic exterior of the two-stranded coiled-coil Pal14Pal21n forces the formation of cyclic tetramers, rather then a polymeric product. It was also found that the conformational “plasticity” of the non-covalent coiled coil Pal14Pal21n is necessary for the formation of the platinum-peptide tetramer. In contrast, the covalently “secured” coiled-coil structure of Pal14C19Pal21nox restricts conformation changes within the molecule, which inhibits the platinum complex from orienting the peptide ligand in order to form the tetramer structure.
Michael Ogawa, (Advisor)
200 p.
de-novo design peptides; coiled-coil; metal-peptide complexes; peptide assemblies; nanoscaled materials

Recommended Citations

Citations

  • Tsurkan, M. V. (2007). Design and Development of Metal-Peptide Nanoscaled Materials [Doctoral dissertation, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1182799840

    APA Style (7th edition)

  • Tsurkan, Mikhail. Design and Development of Metal-Peptide Nanoscaled Materials. 2007. Bowling Green State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1182799840.

    MLA Style (8th edition)

  • Tsurkan, Mikhail. "Design and Development of Metal-Peptide Nanoscaled Materials." Doctoral dissertation, Bowling Green State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1182799840

    Chicago Manual of Style (17th edition)

bgsu1182799840
1,081
© 2007, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.