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The Effect of a 2,2',4,4'-Tetrachlorobiphenyl (PCB 47) and 3,3',4,4'-Tetrachlorobiphenyl (PCB 77) Mixture on Enzymes Involved in the Synthesis of Catecholamines in the Rat Adrenal Gland

Pillai, Mahesh R.
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1214235690

Abstract Details

2008, Master of Science (MS), Bowling Green State University, Biological Sciences.
Polychlorinated biphenyls (PCBs) are environmental pollutants that bioaccumulate through the food web. Lipophilic endocrine disruptors, PCBs accumulate in adipocytes, liver, muscle and breast tissues. The adrenal medulla synthesizes the catecholamines, dopamine, norepinephrine and epinephrine, using several enzymes, including tyrosine hydroxylase (TH) and dopamine β-monooxygenase (DBM). Since catecholamines are important neurotransmitters, this study investigates the effect of PCB 47/77 on DBM and TH expression in adrenal glands of 3, 21 and 60 day old rats. PCB 47/77 was administered orally from conception until the rats were euthanized in doses of 12.5 and 25 ppm. Western blot analyses showed decreased DBM expression in 3 and 60 day old rats treated with both PCB doses, while decreased DBM expression only was observed in 21 day old rats after 25 ppm PCB treatment. TH expression was lower in 3 day old rats treated with 12.5 and 25 ppm PCB as compared to the controls. However, TH expression was elevated in 21 day old rats treated with 12.5 ppm PCB, while in 60 day old rats TH expression was elevated only following 25 ppm PCB treatment. Studies to determine the effect of PCB 47/77 on the developmental expression of DBM and TH showed that in control rats, the greatest DBM and TH levels were observed in 3 day old rats followed by a decrease in expression levels with the increasing age of the rats. Following treatment with 12.5 ppm PCB, DBM expression decreased only in 60 day old rats, while a decrease in 21 and 60 day old rats was observed with 25 ppm PCB treatment as compared to 3 day old rats. In contrast, TH levels were the lowest in 3 day old rats, while a steady increase in TH levels was observed with increasing age of the animals following 25 ppm PCB treatment. Overall, exposure of rat adrenal glands to PCB 47/77 results in alterations in DBM and TH levels that could adversely affect the catecholamine synthesis needed to regulate stress-induced homeostatic mechanisms involved in the flight or fight response.
Tami Steveson, PhD (Advisor)
Lee Meserve, PhD (Committee Member)
Ana Maria Oyarce, PhD (Committee Member)
66 p.
Molecular Biology
PCB; PCB 47/77; catecholamines; dopamine; epinephrine; norepinephrine; tyrosine hydroxylase (TH); dopamine &946; -monooxygenase (DBM); development; dose response

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Citations

  • Pillai, M. R. (2008). The Effect of a 2,2',4,4'-Tetrachlorobiphenyl (PCB 47) and 3,3',4,4'-Tetrachlorobiphenyl (PCB 77) Mixture on Enzymes Involved in the Synthesis of Catecholamines in the Rat Adrenal Gland [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1214235690

    APA Style (7th edition)

  • Pillai, Mahesh. The Effect of a 2,2',4,4'-Tetrachlorobiphenyl (PCB 47) and 3,3',4,4'-Tetrachlorobiphenyl (PCB 77) Mixture on Enzymes Involved in the Synthesis of Catecholamines in the Rat Adrenal Gland. 2008. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1214235690.

    MLA Style (8th edition)

  • Pillai, Mahesh. "The Effect of a 2,2',4,4'-Tetrachlorobiphenyl (PCB 47) and 3,3',4,4'-Tetrachlorobiphenyl (PCB 77) Mixture on Enzymes Involved in the Synthesis of Catecholamines in the Rat Adrenal Gland." Master's thesis, Bowling Green State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1214235690

    Chicago Manual of Style (17th edition)

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© 2008, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.