Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


bgsu1223514067.pdf (1.28 MB)

ETD Abstract Container

Abstract Header

Genetic Mosaicism Between The Bacteriophage φ80 And Bacteriophage λ

Cramer, Todd James
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1223514067

Abstract Details

2008, Master of Science (MS), Bowling Green State University, Biological Sciences.
Mosaicism may occur among the genomes of organisms as well as the viruses or bacteriophage that infect them. Some of these changes result from mutations, insertions, deletions, hybridization, generalized or specialized transduction, transformation, and conjugation. These changes to the genome often lead to strains and/or species different from the original. Comparing sequences of these new forms of the genome to their purported origins can help us further understand the evolutionary links between them and how they may have diverged. As bacteriophage lack ribosomal RNA, and no other universal characteristic exists that allows comparison of many different bacteriophage species to each other, it is clear that genomic and proteomic comparisons will play an invaluable role in the future of bacteriophage phylogenetics. The bacteriophage φ80 has an ~45,000 nucleotide linear dsDNA genome and infects several Gram-negative bacteria, including Escherichia coli, exploiting proteins in the bacteria’s outer membrane for host recognition and the bacterial cell itself for reproduction. The bacteriophage φ80 is a lambdoid bacteriophage, genetically and morphologically related to bacteriophage λ, which also infects E. coli. As φ80 and λ utilize different receptors for host recognition, it is likely that their respective genomes will differ in at least those aspects of the genomes that dictate host range specificity. Many other bacteriophage also infect E. coli, and it is possible that co-infection may occur. When this happens, recombination and reorganization between genomes may occur, creating a new version when viable progeny are released. By comparing the approximately forty percent (40%) of the known sequences of the genome of φ80 to already completed sequences of λ and other lambdoid and non-lambdoid bacteriophage, a clearer understanding of how these two bacteriophage are related has emerged and suggests that they are not as similar as previously assumed. The data presented here suggest that although φ80 is related to λ phylogenetically, its genome has diverged from the genome of λ considerably over a relatively short amount of time.
Ray Larsen (Advisor)
Scott Rogers (Committee Member)
Vipa Phuntumart (Committee Member)
85 p.
Genetics
genetics; mosaicism; bacteriophage; &955; ; &966; 80

Recommended Citations

Citations

  • Cramer, T. J. (2008). Genetic Mosaicism Between The Bacteriophage φ80 And Bacteriophage λ [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1223514067

    APA Style (7th edition)

  • Cramer, Todd. Genetic Mosaicism Between The Bacteriophage φ80 And Bacteriophage λ. 2008. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1223514067.

    MLA Style (8th edition)

  • Cramer, Todd. "Genetic Mosaicism Between The Bacteriophage φ80 And Bacteriophage λ." Master's thesis, Bowling Green State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1223514067

    Chicago Manual of Style (17th edition)

bgsu1223514067
686
© 2008, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.