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Construction and Characterization of Cyanobacterial Bioreporters to Assess Phosphorus Bioavailability in Marine Environments

Nazarov, Alexander N.
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1245347355

Abstract Details

2009, Master of Science (MS), Bowling Green State University, Biological Sciences.
Deficiency of essential nutrients for phytoplankton such as iron and phosphorus is well documented in the world oceans. As a relatively novel approach for identification of bioavailable P in marine systems, this research is focused on the construction and initial characterization of P-responsive cyanobacterial bioreporters for assessing the bioavailability of P in marine environments. Three bioreporters were constructed by transforming the fusion of P-responsive promoter sequences with luxAB into Synechococcus sp. PCC 7002. All obtained bioreporters were shown to produce luminescence after addition of aldehyde; however, the intensity of bioluminescence did not always correlate to the concentration of inorganic phosphate in medium. The analysis of the promoter sequences revealed a similar 15 bp motif that can be a potential consensus sequence conserved in P-responsive promoters of Synechococcus sp. PCC 7002 and some other genetically similar microorganisms.
George S. Bullerjahn, PhD (Advisor)
R. Michael McKay, PhD (Committee Member)
Paul F. Morris, PhD (Committee Member)
34 p.
Molecular Biology; Oceanography
bioreporter; bioavailability; cyanobacteria

Recommended Citations

Citations

  • Nazarov, A. N. (2009). Construction and Characterization of Cyanobacterial Bioreporters to Assess Phosphorus Bioavailability in Marine Environments [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1245347355

    APA Style (7th edition)

  • Nazarov, Alexander. Construction and Characterization of Cyanobacterial Bioreporters to Assess Phosphorus Bioavailability in Marine Environments. 2009. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1245347355.

    MLA Style (8th edition)

  • Nazarov, Alexander. "Construction and Characterization of Cyanobacterial Bioreporters to Assess Phosphorus Bioavailability in Marine Environments." Master's thesis, Bowling Green State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1245347355

    Chicago Manual of Style (17th edition)

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© 2009, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.