Skip to Main Content
 

Global Search Box

 
 
 
 

ETD Abstract Container

Abstract Header

Cross-talk between the TonB and TolA Energy Transduction Systems in Escherichia coli

South, Timothy E.

Abstract Details

2009, Master of Science (MS), Bowling Green State University, Biological Sciences.
The TonB system in Escherichia coli is primarily responsible in providing energy to outer membrane (OM) receptors in order to transport siderophores across the OM and into the periplasm as a means for the cell to acquire iron. The Tol system also provides energy to the OM, ostensibly functioning to maintain membrane integrity. TolA/TonB protein chimeras were made to examine cross-talk between the two systems. While examining cross-talk, specific regions of the TonB protein were determined to be important for energization to occur. While it was known that the transmembrane domain was crucial for energization, data implies that the first 12 residues of TonB also play a significant role in the energization process. A chimera in which the transmembrane domain and the first 12 residues of the N-terminal domain were TolA and the rest of the protein was TonB was able to be energized, and the efficiency at which it worked depended on which components of the TonB and TolA systems were present. A chimera in which only the first 12 residues were TolA and the transmembrane domain and the rest of the protein was TonB did not become energized efficiently, but showed stability no matter which components of the TonB and TolA system were present. An inactive TonB protein is stable in the absence of the other system components, which is one explanation for why the TolA/TonB chimera in which only the first 12 residues are TolA showed stability throughout the study. This data suggested that the first 12 residues of TonB played a crucial role in energizing TonB
Ray Larsen (Advisor)
Scott Rogers (Committee Member)
Jill Zeilstra (Committee Member)
70 p.

Recommended Citations

Citations

  • South, T. E. (2009). Cross-talk between the TonB and TolA Energy Transduction Systems in Escherichia coli [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1249593545

    APA Style (7th edition)

  • South, Timothy. Cross-talk between the TonB and TolA Energy Transduction Systems in Escherichia coli. 2009. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1249593545.

    MLA Style (8th edition)

  • South, Timothy. "Cross-talk between the TonB and TolA Energy Transduction Systems in Escherichia coli." Master's thesis, Bowling Green State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1249593545

    Chicago Manual of Style (17th edition)