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A Single Molecule Study of Calcium Effect on Nuclear Transport

Sarma, Ashapurna
http://orcid.org/0000-0001-7001-1416
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1282326584

Abstract Details

2010, Master of Science (MS), Bowling Green State University, Biological Sciences.
Nuclear pore complexes (NPCs) embedded in the nuclear envelope (NE) is the sole pathway for direct communication between the cytoplasm and the nucleoplasm of eukaryotic cells. NPC allows unregulated passive diffusion of small molecules (< 20 kDa – 40kDa) and facilitated translocation of larger molecules (up to 500 MDa). More and more evidence suggests that calcium stored in the lumen of nuclear envelope and endoplasmic reticulum may further regulate nucleocytoplasmic transport. However with challenges in direct measurements of transport kinetics in the NPC, the calcium-regulated mechanism is still poorly understood. Here single-molecule fluorescence microscopy was used to characterize the nuclear pore permeability of passive diffusion and facilitated translocation under various calcium store concentrations. By snapshots of real-time transient movements of small molecules (10 kDa dextran) and large molecules (97 kDa protein -importin β1 (Imp β), through the NPCs, novel features under real-time trafficking conditions were observed that escaped detection by ensemble measurements. It was found that: i) transport rates cannot be used to reflect the change of nuclear pore permeability, which was mistakenly used in previous ensemble experiments; ii) transport rates for both passive diffusion and facilitated translocation can be significantly affected by the store calcium concentrations; and iii) nuclear pore permeability for passive diffusion is affected more by the amount of stored calcium than that for facilitated translocation.
Dr. Weidong Yang (Advisor)
Dr. Carol Heckman (Committee Member)
Dr. Paul Morris (Committee Member)
72 p.
Biology; Cellular Biology
Nuclear transport; calcium mediated nuclear transport; single-molecule imaging; Nuclear pore complex

Recommended Citations

Citations

  • Sarma, A. (2010). A Single Molecule Study of Calcium Effect on Nuclear Transport [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1282326584

    APA Style (7th edition)

  • Sarma, Ashapurna. A Single Molecule Study of Calcium Effect on Nuclear Transport. 2010. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1282326584.

    MLA Style (8th edition)

  • Sarma, Ashapurna. "A Single Molecule Study of Calcium Effect on Nuclear Transport." Master's thesis, Bowling Green State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1282326584

    Chicago Manual of Style (17th edition)

bgsu1282326584
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© 2010, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.