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Characterization of Three Novel Polyamine Transporters in Plants

Ge, Lingxiao
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1299037220

Abstract Details

2011, Master of Science (MS), Bowling Green State University, Biological Sciences.
Polyamines are low molecular weight aliphatic compounds which are ubiquitous in nature. In plants, polyamines engage in both cellular processes and defenses to environmental stress. In recent years, research on polyamines has focused on biosynthetic and degradative pathways, but no strategy to identify polyamine transport has been employed. In this study one rice gene AK105656 and two Arabidopsis genes At3G19553 and At3G13620 were hypothesized to function as polyamine transporters because of their homologous relationship with known polyamine transporters in several species. The transmembrane structures predicted by several computational tools suggested the probability for them to be membrane transporters. Full length cDNAs of AK105656, At3G19553, and At3G13620 were expressed in the yeast mutant AGP2Δ knockout and analyzed via spot assays. The complementation assays supported the hypothesis that in yeast they were localized to the plasma membrane and functioned as polyamine transporters (importers). Since AK105656 shows close phylogenetic similarity with AtBAT1, which is a bidirectional amino acid transporter in Arabidopsis, AK105656 is also predicted to act as both importer and exporter of polyamines. Further research is still needed to test if AK105656 can also function as a polyamine exporter.
Paul Morris, PhD (Advisor)
Vipa Phuntumart, PhD (Committee Member)
Zhaohui Xu, PhD (Committee Member)
34 p.
Molecular Biology
POLYAMINE TRANSPORTERS; AK105656; AtBAT1; putrescine; yeast

Recommended Citations

Citations

  • Ge, L. (2011). Characterization of Three Novel Polyamine Transporters in Plants [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1299037220

    APA Style (7th edition)

  • Ge, Lingxiao. Characterization of Three Novel Polyamine Transporters in Plants. 2011. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1299037220.

    MLA Style (8th edition)

  • Ge, Lingxiao. "Characterization of Three Novel Polyamine Transporters in Plants." Master's thesis, Bowling Green State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1299037220

    Chicago Manual of Style (17th edition)

bgsu1299037220
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© 2011, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.