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THE EFFECT OF CURCUMIN ON LEWIS LUNG CARCINOMA

Yan, Dejun
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1308588440

Abstract Details

2011, Master of Science (MS), Bowling Green State University, Biological Sciences.
Curcumin, a functional component of the spice turmeric, has a long history of use in Ayurvedic medicine as a treatment for inflammatory conditions. Several biological effects of curcumin in cancer chemoprevention and therapy have been reported and it has been shown to have little adverse effect on normal cells. The present investigation was conducted to determine the effect of curcumin on Lewis lung carcinoma (LLC) cells using both in vivo and in vitro experiments. Specifically, we (1) identified whether curcumin alters the tumorigenicity of LLC cells; (2) evaluated interaction between LLC cells and fibroblast cells to characterize the relationship between cancer cells and tumor stroma; and (3) tested the effect of curcumin in combination with light treatments. To find the effective dosage, curcumin at concentrations between 10 and 60 μM was applied to LLC cells for 30 hours, and then cell density was measured using a standard crystal violet assay. Many cells were eliminated in a dose-dependent manner and 60 μM curcumin was chosen to select for surviving cells that were then used to crate a new cell line. The resulting cell line had lower NF-κB and ALDH1A1 levels and 20% slower growth rate than the original LLC cell line, suggesting that the treated line might contain less aggressive cells. We also injected the treated and untreated cell lines subcutaneously into mice with a C57BL/6 genetic background. The treated line produced fewer tumors than the untreated line, which is consistent with the in vitro results. Histological sections of the tumors confirmed that there was contact between LLC cells and stromal fibroblasts. LLC cells aligned with fibroblasts in co-culture experiments suggesting that they may communicate. When LLC cells were exposed to curcumin and visible light, cell loss was enhanced, confirming previous findings and supporting a therapeutic value of curcumin and light. Our findings further support a role for curcumin as an adjunct to traditional chemo-radiation therapy of lung and possibly other cancers.
Roudabeh J. Jamasbi (Advisor)
Michael E. Geusz (Committee Member)
Vipaporn Phuntumart (Committee Member)
47 p.
Animals; Biochemistry; Biology; Biomedical Research; Biophysics; Cellular Biology; Health Sciences; Medicine; Molecular Biology; Oncology; Pharmacology; Physiology
CURCUMIN; LEWIS LUNG CARCINOMA; CURCUMIN-SURVIVING SUBPOPULATIONS; STROMA; LIGHT

Recommended Citations

Citations

  • Yan, D. (2011). THE EFFECT OF CURCUMIN ON LEWIS LUNG CARCINOMA [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1308588440

    APA Style (7th edition)

  • Yan, Dejun. THE EFFECT OF CURCUMIN ON LEWIS LUNG CARCINOMA. 2011. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1308588440.

    MLA Style (8th edition)

  • Yan, Dejun. "THE EFFECT OF CURCUMIN ON LEWIS LUNG CARCINOMA." Master's thesis, Bowling Green State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1308588440

    Chicago Manual of Style (17th edition)

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© 2011, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.