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Catch of the Day: A yeast One-Hybrid Assay Identifies a Novel DNA-Binding Domain in Phytophthora Sojae

Rutter, Brian Douglas
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1339861904

Abstract Details

2012, Master of Science (MS), Bowling Green State University, Biological Sciences.
Phytophthora sojae is a devastating soybean pathogen. Each year, the voracious activities of this small, oomycete pathogen cost the world billions of dollars. While a large body of research has delved into how P. sojae infects its host, as well as the anatomy and function of effector proteins it uses to promote infection, little is known about the gene regulation coordinating the oomycete's growth, development and virulence. Automated and manual genome annotation approaches have identified more than 1,000 different transcription factors belonging to approximately 60 families in the genomes of six oomycete species: Phytophthora sojae, P. ramorum, P. infestans, Pythium ultimum, Hyaloperonospora. arabidopsidis and Saprolegnia parasitica. Few if any of these putative transcription factors have been verified experimentally. In order to amend this lack of information, a yeast one-hybrid assay was performed with the intent of identifying known and novel transcription factors in P. sojae. Analysis of the promoter regions of the P. sojae genome revealed that approximately 20% of predicted genes in the genome contain the motif ¿¿¿¿¿¿¿GCCGCC,¿¿¿¿¿¿¿ which is a common binding domain for transcription factors in plants. Apicomplexan parasites also regulate their complex life cycles using transcription factors capable of binding to this motif. The assay captured heretofore-unidentified gene sequences that encode a potentially novel DNA-binding domain.
Vipaporn Phuntumart, PhD (Advisor)
Paul Morris, PhD (Committee Member)
Raymond Larsen, PhD (Committee Member)
58 p.
Biology; Molecular Biology
Phytophthora sojae; transcription factor; DNA-binding; yeast one-hybrid

Recommended Citations

Citations

  • Rutter, B. D. (2012). Catch of the Day: A yeast One-Hybrid Assay Identifies a Novel DNA-Binding Domain in Phytophthora Sojae [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1339861904

    APA Style (7th edition)

  • Rutter, Brian. Catch of the Day: A yeast One-Hybrid Assay Identifies a Novel DNA-Binding Domain in Phytophthora Sojae. 2012. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1339861904.

    MLA Style (8th edition)

  • Rutter, Brian. "Catch of the Day: A yeast One-Hybrid Assay Identifies a Novel DNA-Binding Domain in Phytophthora Sojae." Master's thesis, Bowling Green State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1339861904

    Chicago Manual of Style (17th edition)

bgsu1339861904
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© 2012, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.