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Effect of the DNA Methylation Inhibitor 5-aza-2'-Deoxycytidine on the Virulence of the Soybean Pathogen Phytophthora Sojae

Benson, Jennifer

Abstract Details

2012, Master of Science (MS), Bowling Green State University, Biological Sciences.
Phytophthora sojae is an oomycete plant pathogen classified in the Stramenopile kingdom. It specifically targets the soybean crop plant. Infection by P. sojae causes ‘damping off' in seedlings and root rot in older plants. The main form of dispersal is a motile zoospore released when the oomycete is under nutrient or water stress. Diverse gene regulation mechanisms control growth, development, virulence and dispersal of zoospores. Due to the pathogen's ability to rapidly adapt, it is thought that not only genetic but also epigenetic signaling plays a role in P. sojae growth and virulence. The focus of this study is to investigate epigenetic gene regulation mechanisms employed by P. sojae via DNA methylation and its effect on pathogenicity. Treatment with 5-aza-2'-deoxycytidine, a DNA methyltransferase inhibitor, altered hyphal growth rates, zoospore morphology, gene expression, and infection pattern, possibly due to a loss of methylation.
Vipaporn Phuntumart, PhD (Committee Chair)
Paul Morris, PhD (Committee Member)
Raymond Larsen, PhD (Committee Member)
61 p.

Recommended Citations

Citations

  • Benson, J. (2012). Effect of the DNA Methylation Inhibitor 5-aza-2'-Deoxycytidine on the Virulence of the Soybean Pathogen Phytophthora Sojae [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1342052698

    APA Style (7th edition)

  • Benson, Jennifer. Effect of the DNA Methylation Inhibitor 5-aza-2'-Deoxycytidine on the Virulence of the Soybean Pathogen Phytophthora Sojae. 2012. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1342052698.

    MLA Style (8th edition)

  • Benson, Jennifer. "Effect of the DNA Methylation Inhibitor 5-aza-2'-Deoxycytidine on the Virulence of the Soybean Pathogen Phytophthora Sojae." Master's thesis, Bowling Green State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1342052698

    Chicago Manual of Style (17th edition)