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bgsu1350778152.pdf (2.15 MB)
ETD Abstract Container
Abstract Header
The Roles of Moron Genes in the Escherichia Coli Enterobacteria Phage Phi-80
Author Info
Ivanov, Yury V.
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1350778152
Abstract Details
Year and Degree
2012, Doctor of Philosophy (Ph.D.), Bowling Green State University, Biological Sciences.
Abstract
The TonB system couples cytoplasmic membrane-derived proton motive force energy to drive ferric siderophore transport across the outer membrane of Gram-negative bacteria. While much effort has focused on this process, how energy is harnessed to provide for transport of ligands remains unknown. Several bacterial viruses ("phage") are known to require the TonB system to irreversibly adsorb (
i.e.
, establish infection) in the model organism
Escherichia coli
. One such phage is φ80, a "cousin" of the model temperate phage λ. Determining how φ80 is using the TonB system for infection should provide novel insights to the mechanisms of TonB-dependent processes. It had long been known that recombination between λ and φ80 results in a λ-like phage for whom TonB is now required; and this recombination involved the λ J gene, which encodes the tail-spike protein required for irreversible adsorption of λ to
E. coli
. Thus, we suspected that a φ80 homologue of the λ J gene product was responsible for the TonB dependence of φ80. While φ80 has long served as a tool for assaying TonB activity, it has not received the scrutiny afforded λ. Indeed, only a small portion of its genome (not including a J gene homolog) was known. To facilitate the use of φ80 as a molecular tool, we determined and annotated the full genome of the φ80
vir
strain commonly used for TonB studies. The 46,285-bp φ80
vir
genome contained 71 predicted open reading frames, in which the structural genes showed strong synteny with other known lambdoid viruses, with an overall degree of mosaicism indicative of multiple genetic exchanges with other viruses. There was also evidence of unique gene acquisition in the form of morons, one of which, termed
cor
, when cloned and expressed in
E. coli
, blocked TonB-dependent transport of ferric siderophore and irreversible adsorption of φ80
vir
to the TonB-dependent outer membrane protein FhuA. These and other findings reported in this dissertation provide a foundation for the use of φ80
vir
as a tool to dissect the mechanisms of TonB-dependent energy transduction.
Committee
Ray Larsen (Advisor)
Craig Zirbel (Committee Member)
Vipa Phuntumart (Committee Member)
Scott Rogers (Committee Member)
George Bullerjahn (Committee Member)
Pages
104 p.
Subject Headings
Biochemistry
;
Bioinformatics
;
Biology
;
Genetics
;
Microbiology
;
Molecular Biology
;
Virology
Keywords
Phage
;
Phi-80
;
Escherichia coli
;
lysogen
;
TonB
;
FhuA
;
TBDT
;
lipoprotein
;
bacteriophage
;
lambda phage
;
T5 llp
;
lysogenic conversion
;
proton motive force
;
moron gene
;
temperate
;
comparative genomics
;
gene annotation
Recommended Citations
Refworks
EndNote
RIS
Mendeley
Citations
Ivanov, Y. V. (2012).
The Roles of Moron Genes in the Escherichia Coli Enterobacteria Phage Phi-80
[Doctoral dissertation, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1350778152
APA Style (7th edition)
Ivanov, Yury.
The Roles of Moron Genes in the Escherichia Coli Enterobacteria Phage Phi-80.
2012. Bowling Green State University, Doctoral dissertation.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1350778152.
MLA Style (8th edition)
Ivanov, Yury. "The Roles of Moron Genes in the Escherichia Coli Enterobacteria Phage Phi-80." Doctoral dissertation, Bowling Green State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1350778152
Chicago Manual of Style (17th edition)
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Document number:
bgsu1350778152
Download Count:
2,031
Copyright Info
© 2012, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.