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The effects of flavonoids on mitochondrial membrane-associated reduced pyridine nucleotide-utilizing systems of adult Hymenolepis diminuta (cestoda) and Ascaris suum (nematoda)

Shuler, Elizabeth
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1367950138

Abstract Details

2013, Master of Science (MS), Bowling Green State University, Biological Sciences.
The adult intestinal cestode, Hymenolepis diminuta, is energetically anaerobic and displays mitochondria that physiologically function anaerobically. These organelles require an inner membrane-associated NADPH→NAD+ transhydrogenase as well as other membrane-associated, NADH-utilizing activities that reflect anaerobic electron transport. Plant flavonoids are known to affect both mammalian and invertebrate systems and, more specifically, the ecdysone 20-monooxygenase, mitochondrial transhydrogenase and electron transport-linked systems of the tobacco hornworm, Manduca sexta. However, with the exception of the isofavonoid, rotenone, no data existed as to the potential effects of plant flavonoids on parasitic helminth anaerobic, mitochondrial enzymes. Thus, the effects of chrysin, quercetin, morin, juglone, and plumbagin on the H .diminuta transhydrogenase as well as NADH dehydrogenase, NADH-cytochrome c reductase, and an NADH→NAD+ transhydrogenation were evaluated. Although lacking an NADPH→NAD+ system, comparisons with the other corresponding mitochondrial activities of another anaerobic, intestinal, adult helminth, viz., the nematode, Ascaris suum, were made. Activities were assessed spectrophotometrically employing isolated cestode or nematode mitochondrial membranes as the source of enzyme activities. While not all flavonoid treatments proved to significantly affect the activities tested, a suggested inhibition by chrysin was noted and stimulations by juglone and plumbagin were noted for the helminth transhydrogenase. The H. diminuta NADH dehydrogenase was inhibited by plumbagin, but stimulation was apparent at higher concentrations whereas morin stimulated (lower concentrations) and inhibited (higher concentration) and plumbagin stimulated the A. suum enzyme. Both the cestode and nematode NADH-cytochrome c reductase activities appeared to respond positively to the presence of juglone. A tendency towards stimulation of the NADH→NAD+ transhydrogenation of H .diminuta by plumbagin, juglone and chrysin were observed while morin was somewhat inhibitory. In contrast, the A. suum NADH→NAD+ was inhibited by chrysin and juglone, but tended towards stimulation by plumbagin. The study presented apparently represents the first attempts to assess the effects of these plant flavonoids on key mitochondrial systems of the anaerobic, parasitic helminths. Lastly, comparisons of the helminth mitochondrial systems with the mitochondrial, NADPH-dependent ecdysone 20-monooxygenase and the NADPH→NAD+ transhydrogenase systems of M. sexta are made.
Carmen Fioravanti, Dr. (Advisor)
Lee Meserve, Dr. (Committee Member)
Vipaporn Phuntumart, Dr. (Committee Member)
52 p.
Biochemistry; Biology; Parasitology
Hymenolepis diminuta; Ascaris suum; flavonoids; NADPH; NAD; NADH cytochrome c reductase; NADH dehydrogenase; transhydrogenase; transhydrogenation

Recommended Citations

Citations

  • Shuler, E. (2013). The effects of flavonoids on mitochondrial membrane-associated reduced pyridine nucleotide-utilizing systems of adult Hymenolepis diminuta (cestoda) and Ascaris suum (nematoda) [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1367950138

    APA Style (7th edition)

  • Shuler, Elizabeth. The effects of flavonoids on mitochondrial membrane-associated reduced pyridine nucleotide-utilizing systems of adult Hymenolepis diminuta (cestoda) and Ascaris suum (nematoda). 2013. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1367950138.

    MLA Style (8th edition)

  • Shuler, Elizabeth. "The effects of flavonoids on mitochondrial membrane-associated reduced pyridine nucleotide-utilizing systems of adult Hymenolepis diminuta (cestoda) and Ascaris suum (nematoda)." Master's thesis, Bowling Green State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1367950138

    Chicago Manual of Style (17th edition)

bgsu1367950138
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© 2013, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.