Skip to Main Content
 

Global Search Box

 
 
 
 

ETD Abstract Container

Abstract Header

Raman Mode-Selective Spectroscopic Imaging of Redox State in FMN and Flavoprotein

Abstract Details

2014, Master of Science (MS), Bowling Green State University, Chemistry.
Incubated with silver nanoparticles coated with silica, we investigate the surface-enhanced Raman scattering (SERS) spectra of flavin monoucleotide (FMN) in different redox states. Good-quality spectra for oxidized and reduced FMN are obtained at different electrochemical potentials, respectively. Dominate Raman mode shifting occurring at 1623/1610, 1567/1550, 1502/1492 belonging to typical redox-sensitive region are observed and discussed, and they show a good consistency with calculations by using the DFT method. We assign the mode at 1500 cm-1, composed of N5-H bending, N1=C10a stretching and the asymmetric C4a-N5-C5a stretching, as a spectroscopic indicator for the redox state of FMN, since it shows a significant downshift (1502/1492cm-1) and non-linear correlation with potentials when FMN gets reduced from the fully oxidized state using the electrochemical method. Isolated FMN domain from wide type nNOS is studied with the same experimental approach and performs a similar Raman mode down-shifting as pure FMN, further supporting our conclusion that the mode at ~1500cm-1 indicates the redox states for FMN even in a protein environment.
Hong Lu (Advisor)
Ksenija Glusac (Committee Member)
Marshall Wilson (Committee Member)
28 p.

Recommended Citations

Citations

  • Gu, M. (2014). Raman Mode-Selective Spectroscopic Imaging of Redox State in FMN and Flavoprotein [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1398866391

    APA Style (7th edition)

  • Gu, Min. Raman Mode-Selective Spectroscopic Imaging of Redox State in FMN and Flavoprotein. 2014. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1398866391.

    MLA Style (8th edition)

  • Gu, Min. "Raman Mode-Selective Spectroscopic Imaging of Redox State in FMN and Flavoprotein." Master's thesis, Bowling Green State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1398866391

    Chicago Manual of Style (17th edition)