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Revised Jun28-Min Thesis.pdf (952.17 KB)
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Raman Mode-Selective Spectroscopic Imaging of Redox State in FMN and Flavoprotein
Author Info
Gu, Min
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1398866391
Abstract Details
Year and Degree
2014, Master of Science (MS), Bowling Green State University, Chemistry.
Abstract
Incubated with silver nanoparticles coated with silica, we investigate the surface-enhanced Raman scattering (SERS) spectra of flavin monoucleotide (FMN) in different redox states. Good-quality spectra for oxidized and reduced FMN are obtained at different electrochemical potentials, respectively. Dominate Raman mode shifting occurring at 1623/1610, 1567/1550, 1502/1492 belonging to typical redox-sensitive region are observed and discussed, and they show a good consistency with calculations by using the DFT method. We assign the mode at 1500 cm-1, composed of N5-H bending, N1=C10a stretching and the asymmetric C4a-N5-C5a stretching, as a spectroscopic indicator for the redox state of FMN, since it shows a significant downshift (1502/1492cm-1) and non-linear correlation with potentials when FMN gets reduced from the fully oxidized state using the electrochemical method. Isolated FMN domain from wide type nNOS is studied with the same experimental approach and performs a similar Raman mode down-shifting as pure FMN, further supporting our conclusion that the mode at ~1500cm-1 indicates the redox states for FMN even in a protein environment.
Committee
Hong Lu (Advisor)
Ksenija Glusac (Committee Member)
Marshall Wilson (Committee Member)
Pages
28 p.
Subject Headings
Chemistry
Keywords
SERS
;
FMN
;
electrochemical
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Citations
Gu, M. (2014).
Raman Mode-Selective Spectroscopic Imaging of Redox State in FMN and Flavoprotein
[Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1398866391
APA Style (7th edition)
Gu, Min.
Raman Mode-Selective Spectroscopic Imaging of Redox State in FMN and Flavoprotein.
2014. Bowling Green State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1398866391.
MLA Style (8th edition)
Gu, Min. "Raman Mode-Selective Spectroscopic Imaging of Redox State in FMN and Flavoprotein." Master's thesis, Bowling Green State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1398866391
Chicago Manual of Style (17th edition)
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Document number:
bgsu1398866391
Download Count:
703
Copyright Info
© 2014, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.