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Cull Thesis August 2014.pdf (9.55 MB)

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Analysis of Cytosine Methylation in Soybean Pathogen Phytophthora sojae

Cull, Rebecca M
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1404745644

Abstract Details

2014, Master of Science (MS), Bowling Green State University, Biological Sciences.
Phytophthora sojae is plant pathogenic oomycete that targets soybean plants and undergoes a diverse set of life stages specific to its environmental surroundings. Corresponding changes in gene expression occur, although the mechanisms by which genes are differentially expressed and silenced are not fully understood. Methods in regulation of gene expression in P. sojae may include various mechanisms at the transcriptional or post-transcriptional level, including DNA CpG methylation. Among the group of regulated genes in P. sojae are avirulence genes, which encode effector proteins that are utilized during infection. Hundreds of Avr genes are present in the P. sojae genome, although their expression is not uniform across different races. In this study, we investigate the effects of low levels of the cytosine methyltransferase inhibitor 5-aza-2’-deoxycytidine on growth and pathogenicity, in conjunction with qRT-PCR analysis of the housekeeping gene, actin, and the avirulence gene, Avr1a.
Vipaporn Phuntumart, PhD (Advisor)
Paul Morris, PhD (Committee Member)
Raymond Larsen, PhD (Committee Member)
37 p.
Biology; Plant Pathology
cytosine; DNA methylation; soybean; pathogenicity; Phytophthora sojae; oomycete; 5-aza-2-deoxycytidine; avr1a; plant pathogen

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Citations

  • Cull, R. M. (2014). Analysis of Cytosine Methylation in Soybean Pathogen Phytophthora sojae [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1404745644

    APA Style (7th edition)

  • Cull, Rebecca. Analysis of Cytosine Methylation in Soybean Pathogen Phytophthora sojae. 2014. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1404745644.

    MLA Style (8th edition)

  • Cull, Rebecca. "Analysis of Cytosine Methylation in Soybean Pathogen Phytophthora sojae." Master's thesis, Bowling Green State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1404745644

    Chicago Manual of Style (17th edition)

bgsu1404745644
584
© 2014, some rights reserved.Creative Commons License Analysis of Cytosine Methylation in Soybean Pathogen Phytophthora sojae by Rebecca M Cull is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Bowling Green State University and OhioLINK.