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The Coliphage JK5 and the Nature of TonB-Dependence

Kotlarsic, Jennifer L
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1408108270

Abstract Details

2014, Master of Science (MS), Bowling Green State University, Biological Sciences.
Ray Larsen, Advisor JK5 is a novel environmental isolate bacteriophage that was isolated from horse feces. JK5 utilizes the TonB system coupled with the siderophore transporter FhuA for successful infection Escherichia coli cells. This study focused on the characterization of this novel phage and the importance of the J gene that is thought to be responsible for host range specificity. The hypothesis of this study is that the C-terminal region of the J gene is responsibly for both host range specificity and TonB dependence. Plaque assays resulted into a similarity of T1, large clear plaques when plating on laboratory K12 E. coli strain. Similar to T1, JK5 has a genome size of roughly 40,000 base pairs. After genomic comparison, JK5 is similar to RTP as well as T1. After full genomic sequencing, I found that another gene (herein gene38) in addition to the J gene is responsible for host range specificity.
Ray Larsen, Dr. (Advisor)
Vipa Phuntumart, Dr. (Committee Member)
George Bullerjahn, Dr. (Committee Member)
79 p.
Biology; Microbiology; Molecular Biology
Bacteriophage; TonB-Dependence; FhuA

Recommended Citations

Citations

  • Kotlarsic, J. L. (2014). The Coliphage JK5 and the Nature of TonB-Dependence [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1408108270

    APA Style (7th edition)

  • Kotlarsic, Jennifer. The Coliphage JK5 and the Nature of TonB-Dependence . 2014. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1408108270.

    MLA Style (8th edition)

  • Kotlarsic, Jennifer. "The Coliphage JK5 and the Nature of TonB-Dependence ." Master's thesis, Bowling Green State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1408108270

    Chicago Manual of Style (17th edition)

bgsu1408108270
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This open access ETD is published by Bowling Green State University and OhioLINK.