Skip to Main Content
Frequently Asked Questions
Submit an ETD
Global Search Box
Need Help?
Keyword Search
Participating Institutions
Advanced Search
School Logo
Files
File List
Jackson, Cedric James.pdf (794.73 KB)
ETD Abstract Container
Abstract Header
Characterization of Locomotor Response to Psychostimulants in the Parthenogenetic Marbled Crayfish (
Procambarus fallax
forma
virginalis
): A Promising Model for Studying the Epigenetics of Addiction
Author Info
Jackson, Cedric James, Jackson
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1467967151
Abstract Details
Year and Degree
2016, Master of Science (MS), Bowling Green State University, Biological Sciences.
Abstract
CHAPTER I. ABSTRACT : Epigenetic regulation has been implicated as an important factor in understanding the molecular mechanisms of drug addiction. This is due to the life-long behavioral changes that commonly afflict addicted individuals long after drug exposure has been extinguished. Invertebrates, such as crayfish, offer excellent model systems to study these molecular mechanisms because they retain the ancestral neural reward circuit that is evolutionarily conserved across taxa, possess relatively few, large neurons, and have an accessible, modularly organized nervous system. The marbled crayfish (
Procambarus fallax
forma
virginalis
), in particular, has potential as a model for epigenetic studies because it is parthenogenetic, and thus all individuals are genetic clones. To provide the foundation of this model system for parsing the epigenetic mechanisms of drug addiction, here I characterize the locomotor response of juvenile
P. f.
f.
virginalis
exposed to the psychostimulant, d-amphetamine sulfate. Custom video-tracking software was used to record the movement patterns of juveniles exposed to water infused with varying concentrations of d-amphetamine sulfate. ANOVA demonstrated that crayfish locomotion was significantly impacted by drug concentration. These psychostimulant effects, along with the non-invasive mode of drug delivery, which avoids potential epigenetic changes resulting from the stress of direct injection into the hemolymph, set the stage for using
P. f.
f.
virginalis
as an animal model for epigenetic studies. CHAPTER II. ABSTRACT: With the rising interest in using invertebrate models for behavioral epigenetic studies, and the tissue-specific nature of epigenetic changes, there is a need for effective methods of extracting genomic DNA from neural tissue. Invertebrates, such as crayfish, represent useful models in which to parse out the basic underlying epigenetic changes because of the relatively simplified anatomical structure of their nervous system. The parthenogenetic marbled crayfish (
Procambarus fallax
forma
virginalis
) offers a particularly elegant system because it reproduces asexually and produces large numbers of genetically identical offspring. However, the low DNA yield of fatty neural tissue combined with small sample size represent technical challenges in using invertebrate brains. The present study seeks to support the use of the marbled crayfish as a model for studying the mechanisms of behavioral epigenetics by providing an optimized method for genomic DNA extraction from invertebrate neural tissue.
Committee
Moira van Staaden, PhD (Advisor)
Robert Huber, PhD (Committee Member)
Vipaporn Phuntumart, PhD (Committee Member)
Pages
60 p.
Subject Headings
Animal Sciences
;
Behavioral Sciences
;
Behaviorial Sciences
;
Biology
;
Molecular Biology
;
Neurobiology
Keywords
Biology
;
Animal Behavior
;
Addiction
;
Drug Reward
;
Drugs of Abuse
;
Amphetamine
;
Marbled Crayfish
;
Marmokreb
;
epigenetics
;
DNA extraction
Recommended Citations
Refworks
EndNote
RIS
Mendeley
Citations
Jackson, Jackson, C. J. (2016).
Characterization of Locomotor Response to Psychostimulants in the Parthenogenetic Marbled Crayfish (
Procambarus fallax
forma
virginalis
): A Promising Model for Studying the Epigenetics of Addiction
[Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1467967151
APA Style (7th edition)
Jackson, Jackson, Cedric .
Characterization of Locomotor Response to Psychostimulants in the Parthenogenetic Marbled Crayfish (
Procambarus fallax
forma
virginalis
): A Promising Model for Studying the Epigenetics of Addiction.
2016. Bowling Green State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1467967151.
MLA Style (8th edition)
Jackson, Jackson, Cedric . "Characterization of Locomotor Response to Psychostimulants in the Parthenogenetic Marbled Crayfish (
Procambarus fallax
forma
virginalis
): A Promising Model for Studying the Epigenetics of Addiction." Master's thesis, Bowling Green State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1467967151
Chicago Manual of Style (17th edition)
Abstract Footer
Document number:
bgsu1467967151
Download Count:
1,605
Copyright Info
© 2016, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.