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Diel regulation of metabolic functions of a western Lake Erie Microcystis bloom informed by metatranscriptomic analysis

Davenport, Emily J
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1479477147782031

Abstract Details

2016, Master of Science (MS), Bowling Green State University, Biological Sciences.
Despite the global prevalence and apparent increased frequency of freshwater cyanobacterial harmful algal blooms (cHABs), metabolic functions that promote cHAB formation and maintenance remain poorly understood. An opportunity to elucidate cellular responses over diel cycles was presented following the 2014 Toledo (OH, USA) water crisis as part of a 48-h Lagrangian survey following toxin-producing bloom biomass in western Lake Erie. Taxonomic analysis of samples collected from 1 m depth showed the bloom to be dominated by three major phyla: Cyanobacteria (~30%), Proteobacteria (~30%), and Bacteroidetes (~20%). Differential expression analysis of Microcystis reads revealed strong diel patterns of expression between day (10:00 h, 16:00 h) and night (22:00 h, 04:00 h) samples. Day transcripts were abundant in functions related to photosynthesis (e.g. psbA, cpcB), nutrient and energy metabolism (glnA, nirA), cell division (ftsH), heat shock response (dnaK, clpB), and nutrient and ion transporters (bicA, acrB). Night transcripts included microcystin synthesis, amino acid synthesis (carB), and pre-dawn expression of photosynthetic antennae (chlI, cbiE). Hierarchical clustering and PCA showed a uniform grouping of expressed genes at night, whereas day transcripts were separated over the 48-h duration. Lack of uniform clustering of day transcripts suggests that the functional partitioning of gene expression during a Microcystis bloom is not limited to circadian regulation, but also to physico-chemical changes within the environment.
R. Michael McKay, PhD (Advisor)
George Bullerjahn, PhD (Committee Member)
Paul Morris, PhD (Committee Member)
97 p.
Bioinformatics; Biology
Lake Erie; cyanobacteria; Microcystis; microcystin; diel; metatranscriptome; bioinformatics; circadian

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Citations

  • Davenport, E. J. (2016). Diel regulation of metabolic functions of a western Lake Erie Microcystis bloom informed by metatranscriptomic analysis [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1479477147782031

    APA Style (7th edition)

  • Davenport, Emily. Diel regulation of metabolic functions of a western Lake Erie Microcystis bloom informed by metatranscriptomic analysis. 2016. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1479477147782031.

    MLA Style (8th edition)

  • Davenport, Emily. "Diel regulation of metabolic functions of a western Lake Erie Microcystis bloom informed by metatranscriptomic analysis." Master's thesis, Bowling Green State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1479477147782031

    Chicago Manual of Style (17th edition)

bgsu1479477147782031
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This open access ETD is published by Bowling Green State University and OhioLINK.