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The Role Of Gut Microbiome In 3,4 Methylene Dioxymethamphetamine (MDMA) Mediated Hyperthermia In Rats

Choudhury, Sayantan Roy

Abstract Details

2018, Master of Science (MS), Bowling Green State University, Biological Sciences.
The gut microbiome is known to be home to 105 to 106 micro-organisms which are known to be phylogenetically diverse. There is growing evidence that the composition of the gut microbiota plays a large role in the health and well-being of its host. Changes gut microbial populations are associated with the pathogenesis of variety of metabolic, malignant, and inflammatory diseases. Evidence suggests the existence of a `gut-brain axis’ as the involving interactions between gut microbiome and the central nervous system (CNS). Little is known about the involvement of this axis in modulating gut microbial populations in response to drugs of abuse. The present study investigates the effect of 3,4 methylene dioxymethamphetamine (MDMA) on the gut microbiome of male Sprague-Dawley rats. Treatment with MDMA resulted in a rapid compositional shift of the cultivatable bacterial population in the rat cecum. Specifically, the cecal contents of normal MDMA-treated rats yielded a large number of swarming bacteria when cultured on nutrient agar at dilutions suitable for colony counting, whereas organisms with a swarming phenotype were not present at levels detectible in similarly diluted cecal contents from animals that did not receive MDMA. When plated on media containing bile salts, swarming was inhibited, allowing recovery of pure isolates. These isolates were found to be gram-negative rods and non-lactose fermenting rods. Bioinformatic analysis using the 16S rRNA gene from these isolates confirmed then to be members of the genus Proteus.
Vipaporn Phuntumart, Dr. (Advisor)
Raymond Anthony Larsen, Dr. (Committee Member)
Jon Eric Sprague, Dr. (Committee Member)
58 p.

Recommended Citations

Citations

  • Choudhury, S. R. (2018). The Role Of Gut Microbiome In 3,4 Methylene Dioxymethamphetamine (MDMA) Mediated Hyperthermia In Rats [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1530889852900613

    APA Style (7th edition)

  • Choudhury, Sayantan Roy. The Role Of Gut Microbiome In 3,4 Methylene Dioxymethamphetamine (MDMA) Mediated Hyperthermia In Rats. 2018. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1530889852900613.

    MLA Style (8th edition)

  • Choudhury, Sayantan Roy. "The Role Of Gut Microbiome In 3,4 Methylene Dioxymethamphetamine (MDMA) Mediated Hyperthermia In Rats." Master's thesis, Bowling Green State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1530889852900613

    Chicago Manual of Style (17th edition)