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FINAL THESIS JOAN BAUGHMAN-FINAL.pdf (433.46 KB)

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Collecting Low-level DNA Sample of Foreign Contributor From Skin of Known Sources

Baughman, Joan Nicole
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu155567685591486

Abstract Details

2019, Master of Science (MS), Bowling Green State University, Forensic Science.
This study was designed to test the hypothesis that full touch DNA profile of a foreign contributor can be extracted from the skin of a participant. Through trial and error, multiple procedures were performed to attempt to obtain the full profiles. The most efficient method was a combination of the GlobalFiler PCR Amplification Kit, the PCR protocol for the thermal cycler and a modified version of the lysis procedure used in the GlobalFiler PCR Express Amplification Kit. The results throughout were inconsistent, inconclusive and the procedure was unable to be completed properly due to manufacturer level contamination of the GlobalFiler PCR Amplification Kit. New kits need to be purchased and improved modified procedure needs to be replicated. After this occurs, the results should be analyzed and compared as was the previous intention.
Travis Worst (Advisor)
Jon Sprague (Committee Member)
Lewis Maddox (Committee Member)
25 p.
Biology; Molecular Biology
Forensic; Science; Direct; PCR; Touch DNA; low-level DNA; low level DNA; GlobalFiler; DNA; profile; genetic analyzer; genetic analysis; skin; touch DNA from skin; touch DNA off of skin; Direct PCR; Direct; PCR

Recommended Citations

Citations

  • Baughman, J. N. (2019). Collecting Low-level DNA Sample of Foreign Contributor From Skin of Known Sources [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu155567685591486

    APA Style (7th edition)

  • Baughman, Joan. Collecting Low-level DNA Sample of Foreign Contributor From Skin of Known Sources. 2019. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu155567685591486.

    MLA Style (8th edition)

  • Baughman, Joan. " Collecting Low-level DNA Sample of Foreign Contributor From Skin of Known Sources." Master's thesis, Bowling Green State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu155567685591486

    Chicago Manual of Style (17th edition)

bgsu155567685591486
328
© 2019, some rights reserved.Creative Commons License Collecting Low-level DNA Sample of Foreign Contributor From Skin of Known Sources by Joan Nicole Baughman is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Bowling Green State University and OhioLINK.