Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


BGSU_Dissertations_0414_Sun.pdf (5.67 MB)

ETD Abstract Container

Abstract Header

A Cytochemical and Physiological Study of Photosynthetic and Respiratory Electron Reactions in Phormidium Luridum Var. Olivacea

Sun, Donald C.
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566463048506066

Abstract Details

1976, Doctor of Philosophy (Ph.D.), Bowling Green State University, Biological Sciences.
Various cyanobacterial inhibitors and a cyanobacteriocidal toxin produced by Bdellovibrio bacteriovorus (BdAS) were compared for their effect on Phormidium luridum, with regard to morphology, ultrastructure, photosynthesis, respiration, in vitro ferricyanide reduction and electron transport cytochemistry. Electron microscopy reveals a similar cellular response to different cyanobacterial inhibitors, i.e., dichloro-methylurea (DCMU); potassium cyanide (KCN); and BdAS, i.e., formation of intrathylakoidal spaces. At 2000 fc, whole cells of P. luridum produced 405.4 ul 02/mg chl a/hr while fresh spheroplasts produced 326.7 ul 02mg chl a/hr, an efficiency of 80%. The respiration rate of spheroplasts was higher than whole cells.The rate of ferricyanide reduction by whole cells of P. luridum was 126.6 uM/mg chl a/hr. Higher ferricyanide reduction rates were observed in polymyxin B-treated cells (451.2 uM/mg chl a/hr) and spheroplasts (331.2 uM/mg chl a/hr). The ferricyanide reduction rate was stimulated by 0-phenanthroline and BdAS and decreased by DCMU, KCN, ouabain and rotenone.Copper ferrocyanide electron cytochemistry was non-specific except in cells treated with sodium lauryl sulfate. Deposition of 3, 3-diaminobenzidine (DAB) was seen in the intrathylakoidal space. Cytochemical localization, using tetranitroblue tetrazolium (TNBT) was slightly inhibited by BdAS and severely inhibited by ouabain and polymyxin B. Cytochemical results showed that the photosynthetic lamellae of P. luridum also functioned as reactive sites for respiration. The intrathylakoidal space of P. luridum possessed cytochrome c oxidase activity similar to that localized in intracristal spaces of eukaryotic cells. Physiological and cytochemical data indicated that the inhibitory mechanism of BdAS in P. luridum was not identical to other inhibitors used in this study.
Jeffrey C. Burnham (Advisor)
Richard E. Crang (Advisor)
Biology

Recommended Citations

Citations

  • Sun, D. C. (1976). A Cytochemical and Physiological Study of Photosynthetic and Respiratory Electron Reactions in Phormidium Luridum Var. Olivacea [Doctoral dissertation, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566463048506066

    APA Style (7th edition)

  • Sun, Donald. A Cytochemical and Physiological Study of Photosynthetic and Respiratory Electron Reactions in Phormidium Luridum Var. Olivacea. 1976. Bowling Green State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566463048506066.

    MLA Style (8th edition)

  • Sun, Donald. "A Cytochemical and Physiological Study of Photosynthetic and Respiratory Electron Reactions in Phormidium Luridum Var. Olivacea." Doctoral dissertation, Bowling Green State University, 1976. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566463048506066

    Chicago Manual of Style (17th edition)

bgsu1566463048506066
101
This document is provided by Bowling Green State University for research and educational purposes, and may be under copyright by the author or the author's heirs. Please contact the ETD Center administrator at Bowling Green State University etd@bgsu.edu with any questions or comments. In your email, be sure to include the URL and title of the specific items you are inquiring about.
This open access ETD is published by Bowling Green State University and OhioLINK.