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Biological Investigations of Insect Haemocytes

Dailey, Patrick J.
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566463066600033

Abstract Details

1978, Doctor of Philosophy (Ph.D.), Bowling Green State University, Biological Sciences.
Haemocytes from 15 arachnid species and 51 insect species in 12 orders were illustrated, described and classified from stained blood films in the light microscope. In vivo observations were made of haemocytes in the wing veins of Oncopeltus fasciatus, Tenebrio molitor, Acheta domesticus, Blatta orientalis, and Blaberus discoidalis. Observations of living haemocytes in vitro were performed for B. discoidalis, A. domesticus, O. fasciatus, and Gromphadorhina portentosa. The haemocytes of G. portentosa, A. domesticus, T. molitor, and Plodia interpunctella were observed by transmission electron microscopy. Fifty different preparations were tested in fixing cells for scanning electron microscopy (SEM). Differential haemocyte counts (DHC) were made for the majority of species and statistical tests were used to analyze data concerning these counts in selected species. It was found that in most of the species examined the DHC differed significantly between sexes, age groups, and between sexes within age groups. No significant difference was detected in the DHC between two closely related milkweed beetles, Tetraopes tetrophthalmus and T. femoratus. Total haemocyte counts (THC) were made for a few species and their significance was examined. Statistical analyses of the THC between sexes, age groups, between sexes within age groups, and between closely related species did not show significant differences. Cell measurements were made on all cell types described. Cell length/width ratios between the same cell types from stained blood films in T. tetrophthalmus and T. femoratus proved to be significantly different. Haemocytes of B. discoidalis were measured from stained blood films using the light microscope and from scanning electron micrographs of cells prepared for SEM. It was demonstrated that prohaemocytes and plasmatocytes had a greater length and width when measured from stained blood films whereas granular haemocytes were greater in cell width. Spherule cells did not differ significantly in length or width from either stained blood films or scanning electron micrographs.
Robert C. Graves (Advisor)
Biology

Recommended Citations

Citations

  • Dailey, P. J. (1978). Biological Investigations of Insect Haemocytes [Doctoral dissertation, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566463066600033

    APA Style (7th edition)

  • Dailey, Patrick. Biological Investigations of Insect Haemocytes. 1978. Bowling Green State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566463066600033.

    MLA Style (8th edition)

  • Dailey, Patrick. "Biological Investigations of Insect Haemocytes." Doctoral dissertation, Bowling Green State University, 1978. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566463066600033

    Chicago Manual of Style (17th edition)

bgsu1566463066600033
465
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