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The Genetic Basis for Seed Coat Polymorphisms In Lupinus Perennis

Wilson, Rachel Erin
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566754995812035

Abstract Details

2019, Master of Science (MS), Bowling Green State University, Biological Sciences.
Multigenetic traits, specifically seed coat phenotypes, are poorly understood in domesticated plants. This knowledge gap can sometimes be filled by studying wild relatives (Mammadov et al., 2018; von Wettberg 2018). Pigments responsible for seed colors are deposited in the seed coat, which is composed of the two outermost layers of a seed. Seed coat phenotypes can be polymorphic and typically involve complex pathways with multiple layers of expression controlling pigmentation compounds, like anthocyanins. The Anthocyanin Biosynthetic Pathway (ABP) plays a central role in the polymorphic phenotypes of seed coats in many legumes, a family that includes Lupines such as Lupinus perennis (Chalker-Scott, 1999). We hypothesized that the genetics of the ABP correlate with seed coat phenotype. This study aims to identify candidate genes that might be responsible for color differences in the polymorphic seeds of L. perennis. Using RNA sequencing (RNAseq), we produced de novo assemblies of the seed coat transcriptomes of immature seeds of white and speckled seeds. There were two stages of immature seeds used: a pre-pigment and post-pigment stage. The use of both stages increased the chance of constructing a transcriptome containing pigment transcripts. Putative functional annotations of the seed coat transcripts were assigned using multiple databases. Differential expression analysis revealed 58 candidates showing changes in expression patterns correlated between the two phenotypes, involving 36 up expressed and 22 down expressed genes. Two pertain to the ABP and several genes were previously reported to be involved in plant defense, such as Powdery mildew resistance genes. Further work is necessary to verify these results by qPCR and to examine the seed coat transcriptome in greater detail, such as co-expression analysis. These results have important implications for endangered butterfly habitat restoration and crop breeding in the genus Lupinus and suggest that there is much more to understand about these seed coat phenotypes.
Helen Michaels, Dr (Advisor)
Paul Morris, Dr (Committee Member)
Scott Rogers, Dr (Committee Member)
80 p.
Biology; Genetics
Lupinus perennis; RNAseq; Seed coat; Seed polymorphism;

Recommended Citations

Citations

  • Wilson, R. E. (2019). The Genetic Basis for Seed Coat Polymorphisms In Lupinus Perennis [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566754995812035

    APA Style (7th edition)

  • Wilson, Rachel. The Genetic Basis for Seed Coat Polymorphisms In Lupinus Perennis. 2019. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566754995812035.

    MLA Style (8th edition)

  • Wilson, Rachel. "The Genetic Basis for Seed Coat Polymorphisms In Lupinus Perennis." Master's thesis, Bowling Green State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566754995812035

    Chicago Manual of Style (17th edition)

bgsu1566754995812035
491
© 2019, some rights reserved.Creative Commons License The Genetic Basis for Seed Coat Polymorphisms In Lupinus Perennis by Rachel Erin Wilson is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Bowling Green State University and OhioLINK.