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Genome-Scale Metabolic Network Reconstruction of Thermotoga sp. Strain RQ7

Gautam, Jyotshana
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1605228158638208

Abstract Details

2020, Doctor of Philosophy (Ph.D.), Bowling Green State University, Biological Sciences.
Thermotoga are anaerobic hyperthermophiles that have a deep lineage to the last universal ancestor and produce biological hydrogen gas accompanying cell growth. In recent years, systems level approaches have been used to elucidate their metabolic capacities, by integrating mathematical modeling and experimental results. To assist biochemical engineering studies of T. sp. strain RQ7, this work aims at building a metabolic model of the bacterium that quantitatively simulates its metabolism at the genome scale. The constructed model, RQ7_iJG408, consists of 408 genes, 692 reactions, and 538 metabolites. Constraint-based flux balance analyses were used to simulate cell growth in both complex and defined media. Quantitative comparison of the predicted and measured growth rates resulted in good agreements. This model serves as a foundation for an integrated biochemical description of T. sp. strain RQ7. It is a useful tool in designing growth media, identifying metabolic engineering strategies, and exploiting physiological potentials of this biotechnologically significant organism.
Zhaohui Xu (Advisor)
Yuning Fu (Other)
Paul Morris (Committee Member)
Vipa Phuntumart (Committee Member)
Junfeng Shang (Committee Member)
486 p.
Biology; Molecular Biology
Thermotoga; genome-scale metabolic modeling; biomass objective function; constraint-based reconstruction and analysis

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Citations

  • Gautam, J. (2020). Genome-Scale Metabolic Network Reconstruction of Thermotoga sp. Strain RQ7 [Doctoral dissertation, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1605228158638208

    APA Style (7th edition)

  • Gautam, Jyotshana. Genome-Scale Metabolic Network Reconstruction of Thermotoga sp. Strain RQ7 . 2020. Bowling Green State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1605228158638208.

    MLA Style (8th edition)

  • Gautam, Jyotshana. "Genome-Scale Metabolic Network Reconstruction of Thermotoga sp. Strain RQ7 ." Doctoral dissertation, Bowling Green State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1605228158638208

    Chicago Manual of Style (17th edition)

bgsu1605228158638208
255
© 2020, some rights reserved.Creative Commons License Genome-Scale Metabolic Network Reconstruction of Thermotoga sp. Strain RQ7 by Jyotshana Gautam is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Bowling Green State University and OhioLINK.