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Full text release has been delayed at the author's request until January 01, 2026

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An Examination of the Effect of Fraxinus Ash Tree Health on Diversity and Structure of the Soil Microbiome

Ebersole, Wolfgang
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1692372259747792

Abstract Details

2023, Master of Science (MS), Bowling Green State University, Biological Sciences.
Fraxinus ash trees in North America a threatended by the invasive Agrilus planipennis, emerald ash borer (EAB). EAB infestation has wide ranging economic, cultural and ecological impacts upon the North American environment. This study aims to look at the biological impact that infestation of ash trees can have on the soil microbiome around the tree’s roots. A previous study has already examined the possible geochemical effects of the trees sampled, and by looking at the effects on the microbiome through 16S ribosomal sequencing, we hoped to gain some estimation of the effect imposed on microbiota surrounding the tree. With a combination of alpha, beta, and network analysis, we can see some possible changes within the rhizosphere associated to the health of trees. Alpha diversity was inconclusive as there was inconsistent data amongst our sampled set of trees. There is confounding points in the data pertaining to geographic differences in sample locations. However, when stratifying to sample locations, beta and network analyses show shifts in the microbiome are significant, with site 2 and 4 beta diversity showing a progression of microbiome makeup to change as health state changed. Differential networks between health states indicate a change in taxa between healthy and unhealthy tree rhizopheres exist. The network topography as well as the strength of edges between networks showed a difference in taxonomic makeup comparing health states with some phyla of note being Verrucomicrobia, Nitrospirae, and Acidobacteria. Because our sample size within each location was small, we recommend further studies be done to explore those differences.
Zhaohui Xu, Ph.D. (Committee Chair)
Jungfeng Shang, Ph.D. (Committee Member)
Angelica Vazquez Ortega, Ph.D. (Committee Member)
87 p.
Bioinformatics; Biology; Microbiology
Plant microbiome; Soil microbiome; Ash tree; Emerald ash borer; Network analysis; Comparison networks; NetCoMi; Plant soil interaction; Plant health; Microbial phyla;

Recommended Citations

Citations

  • Ebersole, W. (2023). An Examination of the Effect of Fraxinus Ash Tree Health on Diversity and Structure of the Soil Microbiome [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1692372259747792

    APA Style (7th edition)

  • Ebersole, Wolfgang. An Examination of the Effect of Fraxinus Ash Tree Health on Diversity and Structure of the Soil Microbiome . 2023. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1692372259747792.

    MLA Style (8th edition)

  • Ebersole, Wolfgang. "An Examination of the Effect of Fraxinus Ash Tree Health on Diversity and Structure of the Soil Microbiome ." Master's thesis, Bowling Green State University, 2023. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1692372259747792

    Chicago Manual of Style (17th edition)

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© 2023, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.