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Role of RNA structures in the initiation of Rous sarcoma virus reverse transcription

Aiyar, Ashok Anantharaman

Abstract Details

1994, Doctor of Philosophy, Case Western Reserve University, Biochemistry.
Roles of RNA structures in the 5′ non translated region of Rous Sarcoma Virus (RSV) in the initiation of reverse transcription were evaluated. Besides the previously demonstrated U5-leader stem, reverse transcription of RSV was found to require the U5-IR stem. Disruption of the latter by substitution mutations could be compensated by secondary mutations that restored the structure of the stem but not its sequence. Additionally a role for U5 RNA sequences in base-pairing to the TψC loop sequences in primer tRNA Trp was established. This was examined in vivo and in vitro using a substitution mutation that abrogates the U5-TψC interaction. In vitro this mutation could be rescued by using a primer that contained sequences complementary to the substitution. Further studies revealed that these three regions of duplex RNA structure must be maintained at a specific spatial orientation with respect to each other. Disruption of "spacer" sequences between the duplex regions by insertion and deletion mutations caused defects in initiation. In contrast, substitution mutations in these non base-paired spacer sequences had no effect.
Jonathan Leis (Advisor)
187 p.

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Citations

  • Aiyar, A. A. (1994). Role of RNA structures in the initiation of Rous sarcoma virus reverse transcription [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1057948961

    APA Style (7th edition)

  • Aiyar, Ashok. Role of RNA structures in the initiation of Rous sarcoma virus reverse transcription. 1994. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1057948961.

    MLA Style (8th edition)

  • Aiyar, Ashok. "Role of RNA structures in the initiation of Rous sarcoma virus reverse transcription." Doctoral dissertation, Case Western Reserve University, 1994. http://rave.ohiolink.edu/etdc/view?acc_num=case1057948961

    Chicago Manual of Style (17th edition)