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A yeast gene that affects both nuclear import and export

Singleton, David Rivers
http://rave.ohiolink.edu/etdc/view?acc_num=case1061561214

Abstract Details

1994, Doctor of Philosophy, Case Western Reserve University, Pathology.
The present studies have used several approaches to understanding an essential point of eukaryotic gene regulation: what controls the exchange of macromolecules between the nucleus and cytoplasm? Three approaches are presented. In the first, a panel of yeast mutants are identified that accumulate poly-adenylated mRNA within the nucleus at restrictive temperatures, implying a lesion in mRNA export. The genetic basis of an individual mutant is due to a lesion in the MTR13 gene, which codes for an ubiquitous nuclear protein with an RNA binding domain and an extensive arginine-glycine repeat domain. The arginine-glycine repeat domain is similar to domains found in a number of nuclear proteins involved in RNA metabolism. A mutation in the gene was found to block both RNA export and protein import into the nucleus. The mtr13 mutant is the first yeast mutant for which a bidirectional block in nucleocytoplasmic transport has been shown. In the second approach, expression of a gene containing extensive predicted secondary structure has detrimental effects on the growth of otherwise wild-type yeast, suggesting a possible way of screening for suppressor mutations that might be involved in the RNA export pathway. In the third approach, a biochemical method is presented for obtaining nuclear membranes, in order to more carefully characterize the proteins of the inner nuclear envelope
Alan Tartakoff (Advisor)
157 p.
Health Sciences, Pathology
Yeast gene; Nuclear import and export proteins

Recommended Citations

Citations

  • Singleton, D. R. (1994). A yeast gene that affects both nuclear import and export [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1061561214

    APA Style (7th edition)

  • Singleton, David. A yeast gene that affects both nuclear import and export. 1994. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1061561214.

    MLA Style (8th edition)

  • Singleton, David. "A yeast gene that affects both nuclear import and export." Doctoral dissertation, Case Western Reserve University, 1994. http://rave.ohiolink.edu/etdc/view?acc_num=case1061561214

    Chicago Manual of Style (17th edition)

case1061561214
357
© 1994, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.