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Novel Stereolithographic Manufacture of Biodegradable Bone Tissue Scaffolds

Cooke, Malcolm Norman

Abstract Details

2004, Doctor of Philosophy, Case Western Reserve University, Mechanical Engineering.
This research concentrates on both design and manufacturing methodologies for the implementation of stereolithography (SLA) to produce well controlled, custom designed, porous scaffolds in the biodegradable, biocompatible polymer, poly(propylene fumarate) for both in vitro and in vivo studies. Initially, proof of concept trials were undertaken in a custom designed build tank retro-fitted to a standard SLA 250/40 SLA machine to determine stereolithography process feasibility. The results of the initial trials enabled the build process to be scaled-up and implemented on a dedicated Viper Si2 SLA machine. Poly(propylene fumarate) (PPF) and diethyl fumarate (DEF) ratios were adjusted to improve scaffold build quality, which is particularly important for the control of porosity when manufacturing porous scaffolds. Design methodologies are developed that allow three-dimensional porous volumes to be created using hexagonal and plate and post unit cells. Porous scaffolds were designed using this approach by populating a three-dimensional volume from which the scaffold was produced. Two porous scaffold designs were created for an in vitro cell attachment study and for an in vivo toxicity study using a rat dorsal flap animal model. A further set of in vivo animal studies (dog, rat, and rabbit) motivated the design of custom specific porous scaffolds for each animal model. The scaffold designs were based on three-dimensional, computer tomography scan data which was translated and imported into a 3D computer aided design (CAD) environment for the design of porosity to suit each custom scaffold. The CAD data was used to control the SLA process, finally resulting in poly(propylene fumarate), custom porous sterilizable and implantable scaffolds. The preliminary results from the in vitro and in vivo studies are encouraging. This work represents an advancement of knowledge and capability in the design and manufacture of custom porous scaffolds and provides a guide for further research towards the goal of repairing critical-sized cranial defects and other bony defects using tissue engineering technology.
Clare Rimnac (Advisor)
183 p.

Recommended Citations

Citations

  • Cooke, M. N. (2004). Novel Stereolithographic Manufacture of Biodegradable Bone Tissue Scaffolds [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1088797803

    APA Style (7th edition)

  • Cooke, Malcolm. Novel Stereolithographic Manufacture of Biodegradable Bone Tissue Scaffolds. 2004. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1088797803.

    MLA Style (8th edition)

  • Cooke, Malcolm. "Novel Stereolithographic Manufacture of Biodegradable Bone Tissue Scaffolds." Doctoral dissertation, Case Western Reserve University, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=case1088797803

    Chicago Manual of Style (17th edition)