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A Novel Approach to Identification of Diagnositc Markers in Prostate Cancer

Shyshynova, Inna
http://rave.ohiolink.edu/etdc/view?acc_num=case1152729787

Abstract Details

2006, Doctor of Philosophy, Case Western Reserve University, Biochemistry.
Prostate cancer is the most common malignancy and second leading cause of cancer deaths in men. The effectiveness of its cure depends on a timely and accurate diagnosis of the disease as well as application of appropriate therapeutic strategies. We designed an integrated program aimed at identification of prospective marker genes based on their expression properties and applied it to prostate cancer analysis. Our strategy employs in silico expression profiling (an advanced version of human EST database mining) and microarray-based analysis of genes under negative regulation of tumor suppressor genes. A combined list of prospective candidate genes was assembled, which contained a number of known cancer markers, thus demonstrating feasibility of the methodology used. Expression profiles of other candidates were characterized in a large set of tumor cell lines and tumor samples using custom cDNA microarray hybridization, RT-PCR and Northern blots. KIAA1181, MAL2, MGC13170, and FLJ30428, our most promising candidates, were investigated by real-time PCR in prostate cancer and normal tissue samples. KIAA1181 and MAL2 were capable of distinguishing between cancerous and cancer-free prostate as effectively as PSA or PSMA. This approach, proven feasible by the present study, can now be extended to marker identification of virtually any cancer or disease.
Andrei Gudkov (Advisor)
216 p.
Biology, Molecular
prostate cancer marker; tumor suppressor gene; EST data mining; microarray hybridization; prostate specific antigen

Recommended Citations

Citations

  • Shyshynova, I. (2006). A Novel Approach to Identification of Diagnositc Markers in Prostate Cancer [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1152729787

    APA Style (7th edition)

  • Shyshynova, Inna. A Novel Approach to Identification of Diagnositc Markers in Prostate Cancer. 2006. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1152729787.

    MLA Style (8th edition)

  • Shyshynova, Inna. "A Novel Approach to Identification of Diagnositc Markers in Prostate Cancer." Doctoral dissertation, Case Western Reserve University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=case1152729787

    Chicago Manual of Style (17th edition)

case1152729787
1,705
© 2006, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.