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Nonlinear Optical Studies of Potential-sensitive Dyes

LI, HAOWEN
http://rave.ohiolink.edu/etdc/view?acc_num=case1169835690

Abstract Details

2007, Doctor of Philosophy, Case Western Reserve University, Physics.
Di-8-ANEPPS and other similar potential-sensitive dyes have been extensively used to probe the electrical environment in biological membranes due to changes in its linear and nonlinear optical properties as a function of the local electric field. Ratiometric fluorescence, two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) have all been applied to study biological cell and membrane systems. Mechanism and sensitivity studies for these potential-sensitive dyes have become popular due to the increasing biological needs. In this thesis, fluorescence-free time-correlated single photon counting hyper Rayleigh scattering (TCSPC-HRS) has been used to determine the hyperpolarizability Beta. Experiment results are in agreement with quantum chemical calculations. Linear and nonlinear optical studies of di-8-ANEPPS in liquid solution have been carried out. This polarity-dependent solvent effects of linear absorption and hyper-Rayleigh scattering in solution is combined together to analyze the spectral dependence of the sensitivity to the local environment. In this thesis, a two-level model including the spectral shift, changes in transition moment, excited and ground state dipole moment difference, and spectral width is applied to study the sensitivity of di-8-ANEPPS in response to the local electric field (solvent polarity). Good agreement between the model and the measurements has been found. Studies of a di-8-ANEPPS monolayer have been tried and high resolution fluorescence near-field scanning optical microscopic (NSOM) imaging with T-Cells has been performed. Optical resolution beyond diffraction limit has been achieved.
KENNETH SINGER (Advisor)
148 p.
di-8-ANEPPS; hyperpolarizability; Loew; DYES

Recommended Citations

Citations

  • LI, H. (2007). Nonlinear Optical Studies of Potential-sensitive Dyes [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1169835690

    APA Style (7th edition)

  • LI, HAOWEN. Nonlinear Optical Studies of Potential-sensitive Dyes. 2007. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1169835690.

    MLA Style (8th edition)

  • LI, HAOWEN. "Nonlinear Optical Studies of Potential-sensitive Dyes." Doctoral dissertation, Case Western Reserve University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=case1169835690

    Chicago Manual of Style (17th edition)

case1169835690
1,449
© 2007, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.