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case1174263213.pdf (4.16 MB)
ETD Abstract Container
Abstract Header
G-Protein Modulation of Ion Channels and Control of Neuronal Excitability by Light
Author Info
Li, Xiang
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=case1174263213
Abstract Details
Year and Degree
2007, Doctor of Philosophy, Case Western Reserve University, Neurosciences.
Abstract
Many neurotransmitters, hormones and sensory stimuli exert their biological functions through transmembrane receptors which couple to heterotrimeric guanine nucleotide binding protein (G protein) and normally have 7 transmembrane domains called G-protein coupled receptor (GPCR). G proteins work as transducers which convert extracellular signals into intracellular events. Voltage-gated calcium channels (VGCC) (P,Q and N types) and G-protein inwardly rectifying potassium channels (GIRK) are important for modulation of neuronal excitability and synaptic communication. They also belong to the end-targets of GPCRs. These modulations are mediated by G protein âã subunits. Combining electrophysiological recordings and fluorescence resonance energy transfer (FRET), I characterized different regions derived from Gâ2 subunit for interaction with P/Q type calcium channels and GIRK channels. Interestingly, different parts of the Gâ2 subunit can either induce or inhibit G protein modulation of the examined ion channels. In particular, peptides derived from the Gâ2 N-terminus inhibit G protein modulation, whereas peptides derived from Gâ2 C-terminus induced channel modulation. In a second series of studies, I developed light activated probes which could control neuronal excitability. First, I demonstrated that vertebrate rhodopsin can couples to GIRK channels and voltage gated Ca2+ channels through the Gi/o pathway. When expressed in hippocampal neurons, light activation of rhodopsin reduces neuronal firing and modulates synaptic transmission. In contrast, to excite neurons, I applied the green algae channelrhodospin 2 (ChR2), which could generate inward sodium current in HEK293 cells in response to light. Moreover, when ChR2 was expressed in hippocampal neurons, light activation ChR2 can depolarizes neurons to induce action potentials and presynaptic neuronal transmitter release. To further characterize the use of these two light switches in neuronal regulation, I used embryonic chick spinal cords as a model. Electroporated with vertebrate rhodopsin, turning on light could reduce the spontaneous firing and synchronize the bursting activity of spinal cord; on the other hand, light could increase spontaneous activity of spinal cord which was electroporated with ChR2. Thus, with these two probes, I could either increase or decrease neuronal activity with light in a fast and non-invasive way.
Committee
Stefan Herlitze (Advisor)
Pages
179 p.
Subject Headings
Biology, Neuroscience
Keywords
Light
;
neuron
;
G protein
;
rhodopsin
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Citations
Li, X. (2007).
G-Protein Modulation of Ion Channels and Control of Neuronal Excitability by Light
[Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1174263213
APA Style (7th edition)
Li, Xiang.
G-Protein Modulation of Ion Channels and Control of Neuronal Excitability by Light.
2007. Case Western Reserve University, Doctoral dissertation.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=case1174263213.
MLA Style (8th edition)
Li, Xiang. "G-Protein Modulation of Ion Channels and Control of Neuronal Excitability by Light." Doctoral dissertation, Case Western Reserve University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=case1174263213
Chicago Manual of Style (17th edition)
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Document number:
case1174263213
Download Count:
1,057
Copyright Info
© 2007, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.