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MOLECULAR IMAGING OF BREAST CANCER USING PARACEST MRI

Yoo, Byunghee
http://rave.ohiolink.edu/etdc/view?acc_num=case1183658257

Abstract Details

2007, Doctor of Philosophy, Case Western Reserve University, Biomedical Engineering.
In Chapter I. Introduction, two important physiological phenomena in breast cancer, apoptosis and angiogenesis, are described. Two molecular biomarkers, caspases for apoptosis and matrix metalloproteinase’s for angiogenesis, are described particularly in the context of molecular imaging detection methods. In Chapter II. Responsive MR contrast agents, the responsive contrast agents in MR imaging are reviewed based on the response mechanisms, such as tumbling time, water accessibility and water exchange rates. Detecting enzyme activity has wide applications and is a hot issue in molecular imaging research. In Chapter III. Solution phase synthesis of peptidyl contrast agents, a new facile synthetic methodology for creating peptidyl DOTA using an aminoDOTA core compound is described. The solution phase synthesis methodology has limitations for the introduction of diverse sequences of peptides on a DOTA moiety. To overcome this limitation, in Chapter IV. Solid phase synthesis of peptidyl contrast agents, a solid phase synthetic approach was investigated. The solid phase synthetic method has been applied for the synthesis of diverse peptidyl DOTA structures for research presented in this dissertation, and for other molecular imaging research. In Chapter V. Detection of enzyme activity based on PARACESET effect, the newly synthesized caspase-3 targeting peptidyl MR contrast agent was evaluated for the physico/chemical properties. The in vitro enzyme activity detection was demonstrated using caspase-3 and the newly synthesized peptidyl DOTA MR contrast agent. Kinetics studies were conducted by measuring PARACEST effects and the PARACEST kinetics results were compared with the results from fluorescence enzyme kinetics studies. In Chapter VI. Review of DCE-MRI contrast agents, the contrast agents used for DCE-MRI were reviewed according to the criteria of molecular weight. The conventional DCE-MRI method was implemented and tested to show the utility of this technique to study tumor angiogenesis. PARACEST DCE-MRI is new concept of DCE-MRI in many aspects. The PARACEST DCE-MRI technique was studied and the results were compared with conventional T1 DCE-MRI. The results were summarized in Chapter VII. PARACEST DCE-MRI. In Chapter VIII. Future Research, the research directions in the future and tentative projects are suggested.
Mark Pagel (Advisor)
Engineering, Biomedical
contrast agents; PARACEST; MRI; agents; MRI contrast; MRI contrast agents; DCE-MRI

Recommended Citations

Citations

  • Yoo, B. (2007). MOLECULAR IMAGING OF BREAST CANCER USING PARACEST MRI [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1183658257

    APA Style (7th edition)

  • Yoo, Byunghee. MOLECULAR IMAGING OF BREAST CANCER USING PARACEST MRI. 2007. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1183658257.

    MLA Style (8th edition)

  • Yoo, Byunghee. "MOLECULAR IMAGING OF BREAST CANCER USING PARACEST MRI." Doctoral dissertation, Case Western Reserve University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=case1183658257

    Chicago Manual of Style (17th edition)

case1183658257
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© 2007, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.